Amino acid substitutions in the neuraminidase protein of an H9N2 avian influenza virus affect its airborne transmission in chickens

Autor: Jing Lv, Jing Gao, Yumei Cai, Yan Yang, Rong Huang, Huili Yang, Lili Gao, Tongjie Chai, Peiqiang Hou, Airong Wang, Yuwei Gao, Xianzhu Xia, Bingxiao Wang, Wei Liang, Liangmeng Wei
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Veterinary Research
Veterinary Research, BioMed Central, 2015, 46 (1), pp.44. ⟨10.1186/s13567-014-0142-3⟩
ISSN: 0928-4249
1297-9716
DOI: 10.1186/s13567-014-0142-3⟩
Popis: Cases of H9N2 avian influenza virus (AIV) in poultry are increasing throughout many Eurasian countries, and co-infections with other pathogens have resulted in high morbidity and mortality in poultry. Few studies have investigated the genetic factors of virus airborne transmission which determine the scope of this epidemic. In this study, we used specific-pathogen-free chickens housed in isolators to investigate the airborne transmissibility of five recombinant H9N2 AIV rescued by reverse genetic technology. The results show that airborne transmission of A/Chicken/Shandong/01/2008 (SD01) virus was related to the neuraminidase (NA) gene, and four amino acid mutations (D368E, S370L, E313K and G381D) within the head region of the SD01 NA, reduced virus replication in the respiratory tract of chickens, reduced virus NA activity, and resulted in a loss of airborne transmission ability in chickens. Similarly, reverse mutations of these four amino acids in the NA protein of r01/NASS virus, conferred an airborne transmission ability to the recombinant virus. We conclude that these four NA residues may be significant genetic markers for evaluating potential disease outbreak of H9N2 AIV, and propose that immediate attention should be paid to the airborne transmission of this virus. Electronic supplementary material The online version of this article (doi:10.1186/s13567-014-0142-3) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
Externí odkaz: