Induction of Type-Specific Neutralizing Antibodies by Capsomeres of Human Papillomavirus Type 33
| Autor: | Martin Sapp, Tzenan Giroglou, Rolf E. Streeck, Claudia Fligge |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_treatment
Dose-Response Relationship Immunologic Enzyme-Linked Immunosorbent Assay Antibodies Viral papillomavirus Neutralization Mice Capsid Neutralization Tests dose response Virology medicine Animals Humans Papillomaviridae Antiserum Mice Inbred BALB C biology Immunogenicity Capsomere Virion neutralization vaccination Titer biology.protein capsomeres Immunization Antibody Adjuvant |
| Zdroj: | Virology. 283:353-357 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.2000.0875 |
| Popis: | The immunogenicity of capsomeres of human papillomavirus type 33 was evaluated in a dose–response analysis. Capsomeres were obtained free of capsids by expression of L1 carrying the single point mutation C427S. Neutralizing antibodies were detected using an in vitro pseudoinfection assay. Capsomeres induced type-specific, neutralizing antibodies in mice even in the absence of adjuvant. The neutralization titers of immune sera raised without adjuvant were 10- to 20-fold lower than those of antisera to virus-like particles, but virtually identical using Freund's adjuvant. These data indicate that capsomeres may substitute for virus-like particles in future vaccines when used with an adjuvant appropriate for human vaccination. |
| Databáze: | OpenAIRE |
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