Mapping of crown rust resistance gene Pc53 in oat (Avena sativa)

Autor: Kathy Esvelt Klos, J. Michael Bonman, Belayneh Admassu-Yimer
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Avena
Genetic Linkage
01 natural sciences
Puccinia coronata
Genotype
Disease Resistance
Plant Proteins
Genetics
Multidisciplinary
biology
Nucleotide Mapping
Chromosome Mapping
Eukaryota
Agriculture
Plants
Phenotype
Experimental Organism Systems
Medicine
Oat
Genome
Plant
Research Article
Science
Quantitative Trait Loci
Single-nucleotide polymorphism
Quantitative trait locus
Research and Analysis Methods
Polymorphism
Single Nucleotide
Molecular Genetics
03 medical and health sciences
Gene mapping
Genetic linkage
Plant and Algal Models
Plant breeding
Grasses
Molecular Biology Techniques
Linkage Mapping
Gene
Molecular Biology
Plant Diseases
Gene Mapping
Organisms
Biology and Life Sciences
biology.organism_classification
Agronomy
Plant Breeding
030104 developmental biology
Seedlings
Animal Studies
Rice
010606 plant biology & botany
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 12, p e0209105 (2018)
ISSN: 1932-6203
Popis: Crown rust disease caused by the fungus Puccinia coronata f. sp. avenae (Pca) is a major production constraint of oat in North America, Europe, and Australia. There are over 100 genes effective against one or more Pca races, but only a handful of seedling resistance (Pc) genes have been mapped to a known chromosomal location. The goal of the present study was to use linkage mapping to identify the genomic location of the Pc53 gene, and to produce a list of linked SNPs with potential as molecular markers for marker assisted breeding. The Pc53 gene was placed on the linkage group Mrg08 at 82.4 cM using F5-derived recombinant inbred lines (RILs) from a cross between the Pc53 carrier 6-112-1-15 (PI 311624) and the susceptible cultivar Otana. The map location was validated using RILs from a cross between 6-112-1-15 and the Pc50 differential line. Single nucleotide polymorphism marker GMI_ES02_c14533_567 was the closest to Pc53. A major seedling resistance gene 'PcKM' and QTL QcC.Core.08.1, QCr.Core.08.2, QCr.Core.08.3 and QCr.cdl9-12D were previously reported on Mrg08. QPc.Core.08.1 and PcKM were mapped to within 1 cM of Pc53; but previous virulence studies have indicated separate identities. The chromosomal location of Pc53 and SNPs linked with it will facilitate the utilization of Pc53 in oat breeding programs.
Databáze: OpenAIRE
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