Alternative identification test relying upon sexual reproductive abilities of Candida lusitaniae strains isolated from hospitalized patients
| Autor: | Jean Villard, Anne Favel, Annie Brulfert, Fabienne François, Régis Pépin, Thierry Noël, Christiane Chastin |
|---|---|
| Přispěvatelé: | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Mating type Auxotrophy [SDV]Life Sciences [q-bio] Mycology 03 medical and health sciences Meiosis Humans Mating Mycological Typing Techniques Crosses Genetic ComputingMilieux_MISCELLANEOUS Candida 030304 developmental biology Genetics 0303 health sciences biology Strain (chemistry) 030306 microbiology Candida lusitaniae Candidiasis Fungi imperfecti biology.organism_classification Culture Media 3. Good health Hospitalization Microscopy Electron Ascospore |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2001, 39 (11), pp.3906-3914. ⟨10.1128/JCM.39.11.3906-3914.2001⟩ |
| ISSN: | 0095-1137 |
| Popis: | The in vitro mating ability of Candida lusitaniae (teleomorph Clavispora lusitaniae ) clinical isolates has been investigated. Studying the effects of culture conditions, we showed that ammonium ion depletion in the medium is a major trigger of the sexual cycle. Moreover, a solid support is required for mating, suggesting a role for adhesion factors in addition to the mating type gene recognition function. Monitoring of mating and meiosis efficiency with auxotrophic strains showed great variations in ascospore yields, which appeared to be strain and temperature dependent, with an optimal range of 18 to 28°C. The morphogenetic events taking place from mating to ascospore release were studied by scanning and electron microscopy, and the ultrastructure of the conjugation canal, through which intercellular nuclear exchanges occur, was revealed. Labeling experiments with a lectin-fluorochrome system revealed that the nuclear transfer was predominantly polarized, thus allowing a distinction between the nucleus donor and the nucleus acceptor strains. The direction of the transfer depended on the strain combination used, rather than on the genotypes of the strains, and did not appear to be controlled by the mating type genes. Finally, we demonstrated that all of the 76 clinical isolates used in this study were able to reproduce sexually when mated with an opposite mating type strain, and we identified a 1:1 MAT a/ MAT α ratio in the collection. These results support the idea that there is no anamorph state in C. lusitaniae . Accordingly, the mating type test, which is easy to use and can usually be completed within 48 h, is a reliable alternative identification system for C. lusitaniae . |
| Databáze: | OpenAIRE |
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