Ivermectin also inhibits the replication of bovine respiratory viruses (BRSV, BPIV-3, BoHV-1, BCoV and BVDV) in vitro
| Autor: | Eda Baldan Toker, Kadir Yeşilbağ, Ozer Ates |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Bovine parainfluenza type 3 virus
Cancer Research medicine.drug_class viruses Drug Evaluation Preclinical Bovine Respiratory Disease Complex Virus Attachment Bovine respiratory disease Biology Virus Replication Antiviral Agents Article Bovine herpesvirus type 1 Virus Madin Darby Canine Kidney Cells 03 medical and health sciences Bovine respiratory syncytial virus Dogs Ivermectin Bovine coronavirus Virology medicine Animals RNA Viruses 030304 developmental biology 0303 health sciences urogenital system 030306 microbiology RNA medicine.disease Antiviral efficiency In vitro Titer Infectious Diseases In vitro testing embryonic structures Cattle Antiviral drug Bovine viral diarrhea virus medicine.drug |
| Zdroj: | Virus Research |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2021.198384 |
| Popis: | Bovine respiratory disease (BRD) complex is an important viral infection that causes huge economic losses in cattle herds worldwide. However, there is no directly effective antiviral drug application against respiratory viral pathogens; generally, the metaphylactic antibacterial drug applications are used for BRD. Ivermectin (IVM) is currently used as a broad-spectrum anti-parasitic agent both for veterinary and human medicine on some occasions. Moreover, since it is identified as an inhibitor for importin α/β-mediated nuclear localization signal (NLS), IVM is also reported to have antiviral potential against several RNA and DNA viruses. Since therapeutic use of IVM in COVID-19 cases has recently been postulated, the potential antiviral activity of IVM against bovine respiratory viruses including BRSV, BPIV-3, BoHV-1, BCoV and BVDV are evaluated in this study. For these purposes, virus titration assay was used to evaluate titers in viral harvest from infected cells treated with non-cytotoxic IVM concentrations (1, 2.5 and 5 μM) and compared to titers from non-treated infected cells. This study indicated that IVM inhibits the replication of BCoV, BVDV, BRSV, BPIV-3 and BoHV-1 in a dose-dependent manner in vitro as well as number of extracellular infectious virions. In addition, it was demonstrated that IVM has no clear effect on the attachment and penetration steps of the replication of the studied viruses. Finally, this study shows for the first time that IVM can inhibit infection of BRD-related viral agents namely BCoV, BPIV-3, BVDV, BRSV and BoHV-1 at the concentrations of 2.5 and 5 μM. Consequently, IVM, which is licensed for antiparasitic indications, also deserves to be evaluated as a broad-spectrum antiviral in BRD cases caused by viral pathogens. |
| Databáze: | OpenAIRE |
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