Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts

Autor: María Mittelbrunn, Francesc Baixauli, Angel Luis Jaso-Tamame, Inmaculada Jorge, Johan Garaude, Gloria González-Aseguinolaza, Noa B. Martín-Cófreces, Miguel Vicente-Manzanares, José Antonio Enríquez, Salvador Iborra, Carolina Villarroya-Beltri, Irene Fernández-Delgado, Rebeca Acín-Pérez, Ana Latorre-Pellicer, Francisco Sánchez-Madrid, Daniel Torralba
Přispěvatelé: Ministerio de Economía y Competitividad (España), Comunidad de Madrid, Centro de Investigación Biomedica en Red - CIBER, European Commission, Instituto de Salud Carlos III, Fundación ProCNIC, European Regional Development Fund, Centro Nacional de Investigaciones Cardiovasculares (España), UAM. Departamento de Medicina, Instituto de Investigación del Hospital de La Princesa (IP)
Rok vydání: 2018
Předmět:
0301 basic medicine
Dendritic cells (DC
IMMUNOLOGICAL SYNAPSE
Contacts
MITOCHONDRIAL-DNA
T-Lymphocytes
General Physics and Astronomy
Priming (immunology)
Gene Expression
Lymphocyte Activation
Jurkat cells
Jurkat Cells
Interferon
lcsh:Science
Cells
Cultured
IN-VIVO
Mice
Knockout
Multidisciplinary
Chemistry
IMMUNE-RESPONSES
Extracellular vesicles
3. Good health
Cell biology
medicine.anatomical_structure
Viruses
medicine.drug
Anti-pathogenic programs
Medicina
T cell
Science
chemical and pharmacologic phenomena
KAPPA-B
INNATE
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Immune system
Antigen
Cell Line
Tumor
Dendritic cells (DC)
medicine
Animals
Humans
Antigens
CANCER-CELLS
EXOSOMES
RELEASE
Physiological consequences
IDENTIFICATION
HEK 293 cells
General Chemistry
Dendritic Cells
DNA
Mice
Inbred C57BL
030104 developmental biology
HEK293 Cells
Cell culture
lcsh:Q
Interferons
Zdroj: Repisalud
Instituto de Salud Carlos III (ISCIII)
Biblos-e Archivo. Repositorio Institucional de la UAM
instname
Digital.CSIC. Repositorio Institucional del CSIC
Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid
Consejería de Sanidad de la Comunidad de Madrid
Nature Communications, Vol 9, Iss 1, Pp 1-17 (2018)
Nature Communications
Popis: Interaction of T cell with antigen-bearing dendritic cells (DC) results in T cell activation, but whether this interaction has physiological consequences on DC function is largely unexplored. Here we show that when antigen-bearing DCs contact T cells, DCs initiate anti-pathogenic programs. Signals of this interaction are transmitted from the T cell to the DC, through extracellular vesicles (EV) that contain genomic and mitochondrial DNA, to induce antiviral responses via the cGAS/STING cytosolic DNA-sensing pathway and expression of IRF3-dependent interferon regulated genes. Moreover, EV-treated DCs are more resistant to subsequent viral infections. In summary, our results show that T cells prime DCs through the transfer of exosomal DNA, supporting a specific role for antigen-dependent contacts in conferring protection to DCs against pathogen infection. The reciprocal communication between innate and adaptive immune cells thus allow efficacious responses to unknown threats.
Ministry of Economy and Competitiveness, CAM S2017/BMD-3671 from the Comunidad de Madrid, CIBER Cardiovascular (Fondo de Investigación Sanitaria del Instituto de Salud Carlos III and co-funding by Fondo Europeo de Desarrollo Regional FEDER), ERC-2011-AdG 294340-GENTRIS and COST-Action BM1202 to F.S.-M.; grant SAF2015-65633-R from the Spanish Ministry of Economy and Competitiveness to J.A.E. M.M. is supported by MS14/00219 from Instituto de Salud Carlos III. Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the Spanish Ministry of Economy and Competitiveness (MINECO)
Databáze: OpenAIRE
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