The basic helix-loop-helix factor Hand2 regulates autonomic nervous system development
| Autor: | Jianming Hao, Peter Cserjesi, Yan-Shan Dai, Sunny Hwang, Christopher P. Bonin, Yuka Morikawa |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
animal structures Transcription Genetic P300-CBP Transcription Factors Biology Autonomic Nervous System Article Mice Cell Line Tumor Internal medicine Basic Helix-Loop-Helix Transcription Factors medicine Animals p300-CBP Transcription Factors Transcription factor Mice Knockout Regulation of gene expression Histone Acetyltransferase p300 Basic helix-loop-helix Helix-Loop-Helix Motifs Gene Expression Regulation Developmental food and beverages Cell biology Autonomic nervous system Endocrinology embryonic structures biology.protein Ectopic expression HAND2 Developmental Biology |
| Zdroj: | Developmental Dynamics. 234:613-621 |
| ISSN: | 1058-8388 |
| Popis: | Mammalian autonomic nervous system (ANS) development requires the combinatorial action of a number of transcription factors, which include Mash 1, Phox 2b, and GATA 3. Here we show that the bHLH transcription factor, Hand 2 (dHAND), is expressed concurrently with Mash 1 during sympathetic nervous system (SNS) development and that the expression of Hand 2 is not dependent on Mash 1. This suggests that these two bHLH factors work in parallel during SNS development. We also show that ectopic expression of Hand 2 activates the neuronal program and promotes the acquisition of a phenotype corresponding to peripheral neurons including neurons of the SNS lineage in P19 embryonic carcinoma cells. We propose that Hand 2 works in parallel with other members of the transcriptional network to regulate ANS developmental but can ectopically activate the program by a cross-regulatory mechanism that includes the activation of Mash 1. We show that this function is dependent on its interaction with the histone acetyltransferase p300/CBP, indicating that Hand 2 functions to promote ANS development as part of a larger transcriptional complex. |
| Databáze: | OpenAIRE |
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