Detection of multiple viral sequences in the respiratory tract samples of suspected Middle East respiratory syndrome coronavirus patients in Jakarta, Indonesia 2015-2016

Autor: Aghnianditya Kresno Dewantari, Teguh Sarry Hartono, Tri Yuli Setianingsih, Ageng Wiyatno, Khin Saw Aye Myint, Rosamarlina, Dodi Safari, Vivi Lisdawati, Evi Hindawati
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
viruses
Respiratory System
Dengue virus
medicine.disease_cause
MERS-CoV
0302 clinical medicine
030212 general & internal medicine
Viral
Coronavirus
Travel
biology
virus diseases
General Medicine
respiratory system
Middle Aged
Hospitalization
Infectious Diseases
PCR
Viruses
RNA
Viral
Female
Rhinovirus
Coronavirus Infections
Microbiology (medical)
Adult
Human coronavirus 229E
Middle East respiratory syndrome coronavirus
030106 microbiology
Virus
Article
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Middle East
Human metapneumovirus
medicine
Humans
lcsh:RC109-216
Aged
business.industry
Sequence Analysis
RNA
Pneumonia
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Virology
respiratory tract diseases
Indonesia
Enterovirus
business
Zdroj: International Journal of Infectious Diseases
International Journal of Infectious Diseases, Vol 86, Iss, Pp 102-107 (2019)
ISSN: 1878-3511
Popis: Highlights • Nine viral etiological agents were identified from 13 MERS-CoV patients with pneumonia in Indonesia from 2015- to 2016. • Patients with lower respiratory infections and travel history to Middle East should be screened for other respiratory viruses in addition to MERS-CoV. • This study confirmed that there was no MERS-CoV in the 13 suspected MERS-CoV patients with pneumonia.
Objectives The identification and analysis of viral etiological agents from suspected Middle East respiratory syndrome coronavirus (MERS-CoV) cases admitted to Prof. Dr. Sulianti Saroso Infectious Disease Hospital (IDH) using molecular assays. Methods Biological samples were collected from 13 hospitalized patients suspected of MERS-CoV infection in Prof. Dr. Sulianti Saroso IDH from July 2015 to December 2016. The majority of patients presented with pneumonia, with symptoms including fever (≥37.5 °C), labored breathing, and cough, and with a history of travel to the Middle East. Viral RNA was isolated and converted to cDNA, which was used as a template for the detection of 12 viral panels using conventional PCR and sequencing. Results Viral etiological agents detected in the patients were enterovirus D68, dengue virus type 3, rhinovirus C, human coronavirus 229E, herpes simplex virus type 1, influenza virus H1N1, influenza virus H3N2, human metapneumovirus, and rhinovirus A60. Conclusions The sequences of nine viral agents under different taxa were detected in suspected MERS-CoV patients, including influenza virus, paramyxovirus, coronavirus, enterovirus, human metapneumovirus, and herpesvirus.
Databáze: OpenAIRE
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