THE EXPRESSION OF THE CYTOMEGALOVIRUS CHEMOKINE RECEPTOR HOMOLOG US28 SEQUESTERS BIOLOGICALLY ACTIVE CC CHEMOKINES AND ALTERS IL-8 PRODUCTION
| Autor: | Beverly Torok-Storb, Julie-Randoph Randolph-Habecker, Pappachan E. Kolattukudy, Daniel D. Sedmak, Brad H. Rovin, Brian M. Rahill, Jeffrey Vieira |
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| Rok vydání: | 2002 |
| Předmět: |
Umbilical Veins
Immunology Cytomegalovirus Enzyme-Linked Immunosorbent Assay C-C chemokine receptor type 6 Biology CCL7 Biochemistry Monocytes Viral Proteins Chemokine receptor Humans Immunology and Allergy CCL17 CXC chemokine receptors CX3CL1 Chemokine CCL5 Molecular Biology Cells Cultured Inflammation Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Chemotaxis Interleukin-8 virus diseases Hematology Blotting Northern Cell biology CXCL2 Chemokines CC Receptors Chemokine Endothelium Vascular Chemokines Protein Binding CCL21 |
| Zdroj: | Cytokine. 19:37-46 |
| ISSN: | 1043-4666 |
| DOI: | 10.1006/cyto.2002.0874 |
| Popis: | We hypothesized that US28, a cytomegalovirus (CMV) CC chemokine receptor homolog, plays a role in modulating the host antiviral defense. Monocyte chemotaxis was induced by supernatants from fibroblasts infected with a US28 deletion mutant of CMV (CMV Delta US28) due to endogenously produced CC chemokines MCP-1 and RANTES. However, these chemokines were sequestered from the supernatants of CMV-infected cells that did express US28. US28 was also capable of sequestering exogenously added RANTES. Surprisingly, cells infected with CMV Delta US28 transcribed and secreted increased levels IL-8, a CXC chemokine, when compared to CMV-infected cells. Finally, because chemokines are potent mediators of immune cell migration through the endothelium, we characterized the CC chemokine binding potential of CMV-infected endothelial cells. We propose that US28 functions as a 'chemokine sink' by sequestering endogenously and exogenously produced chemokines and alters the production of the CXC chemokine IL-8, suggesting that CMV could significantly alter the inflammatory milieu surrounding infected cells. |
| Databáze: | OpenAIRE |
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