Leukaemia inhibitory factor enhances sheep fertilization in vitro via an influence on the oocyte
| Autor: | K. Matsukawa, Grazyna Ptak, Marian Tischner, Pasqualino Loi, Federica Lopes |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Cell Survival Cleavage Stage Ovum media_common.quotation_subject medicine.medical_treatment Parthenogenesis Embryonic Development Fertilization in Vitro Biology Leukemia Inhibitory Factor Human fertilization Food Animals Internal medicine medicine Animals Sexual Maturation Blastocyst Small Animals Ovulation Cells Cultured reproductive and urinary physiology media_common Sheep In vitro fertilisation Interleukin-6 urogenital system Equine Embryogenesis Embryo Oocyte Spermatozoa medicine.anatomical_structure Endocrinology embryonic structures Oocytes Oviduct Female Animal Science and Zoology hormones hormone substitutes and hormone antagonists |
| Zdroj: | Theriogenology. 65:1891-1899 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2005.10.018 |
| Popis: | LIF is twice transiently expressed in the mouse uterus, first at the time of ovulation and again just prior to implantation, and studies have demonstrated a beneficial influence of this cytokine on embryo development in several species. We have investigated the effect of LIF on gametes in vitro, on the hypothesis that the ovulatory peak of LIF can exert an influence on gametes present within the oviduct. We also investigated the effect of LIF on in vitro fertilization and embryo development, in oocytes from adult sheep and from prepubertal lambs that lack the preovulatory hormone surge and that are unable to sustain early embryonic development. A higher rate of pronuclear-stage embryos derived from both, adult and prepubertal female, was obtained when in vitro fertilization was performed in the presence of LIF, and there was an improved cleavage of parthenogenetic embryos when incubated with LIF immediately following activation. In contrast, LIF was found to have no influence on the viability of ram semen. In vitro fertilized two-cell stage embryos from adult sheep and prepubertal lambs, cultured in defined medium enriched with LIF, both reached the blastocyst stage at similar rates to control embryos. However, LIF exerted a positive influence on the quality of the blastocysts as revealed by significantly higher number of ICM cells and total number of cells. Together, these data demonstrate that LIF exerts a beneficial effect on sheep oocytes and embryos in vitro, but only at stages concomitant with steroid hormones surges. |
| Databáze: | OpenAIRE |
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