An inducible potato ( E,E )‐farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco

Autor: Varun Dwivedi, Sarma Rajeev Kumar, H. B. Shilpashree, Ram Krishna, Srinivas Rao, Ajit K. Shasany, Shannon B. Olsson, Dinesh A. Nagegowda
Rok vydání: 2022
Předmět:
Zdroj: The Plant Journal. 111:1308-1323
ISSN: 1365-313X
0960-7412
DOI: 10.1111/tpj.15890
Popis: Terpene synthases (TPSs) have diverse biological functions in plants. Though the roles of TPSs in herbivore defense are well established in many plant species, their role in bacterial defense has been scarce and is emerging. Through functional genomics, here we report the in planta role of potato (Solanum tuberosum) terpene synthase (StTPS18) in bacterial defense. Expression of StTPS18 was highest in leaves and was induced in response to Pseudomonas syringae and methyl jasmonate treatments. The recombinant StTPS18 exhibited bona fide (E,E)-farnesol synthase activity forming a sesquiterpenoid, (E,E)-farnesol as the sole product, utilising (E,E)-farnesyl diphosphate (FPP). Subcellular localization of GFP fusion protein revealed that StTPS18 is localized to the cytosol. Silencing and overexpression of StTPS18 in potato resulted in reduced and enhanced tolerance, respectively, to bacterial pathogens P. syringae and Ralstonia solanacearum. Bacterial growth assay using medium containing (E,E)-farnesol significantly inhibited P. syringae growth. Moreover, StTPS18 overexpressing transgenic potato and Nicotiana tabacum leaves, and (E,E)-farnesol and P. syringae infiltrated potato leaves exhibited elevated expression of sterol pathway and members of pathogenesis-related genes with enhanced phytosterol accumulation. Interestingly, enhanced phytosterols in
Databáze: OpenAIRE
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