Expression of endothelin-converting enzyme, endothelin-1 and endothelin receptors at the site of percutaneous coronary intervention in humans

Autor: Takahiko Naruko, Kazuo Haze, Minoru Yoshiyama, Yoshimi Sugama, Takeshi Inoue, Masahiko Ohsawa, Akira Itoh, Shoichi Ehara, Chizuko Kitabayashi, Yoshihiro Ikura, Junichi Yoshikawa, Kazuhiko Tanzawa, Makiko Ueda, Nobuyuki Shirai, Michihiko Hirayama
Rok vydání: 2006
Předmět:
Atherectomy
Coronary
Male
medicine.medical_specialty
Physiology
Endothelin converting enzyme 1
Myocytes
Smooth Muscle
Endothelin-Converting Enzymes
Pathogenesis
Restenosis
Internal medicine
Internal Medicine
medicine
Aspartic Acid Endopeptidases
Humans
Myocyte
Angioplasty
Balloon
Coronary
education
Receptor
Aged
Aged
80 and over
education.field_of_study
Endothelin-1
Receptors
Endothelin
business.industry
Metalloendopeptidases
Middle Aged
respiratory system
Receptor
Endothelin A
musculoskeletal system
medicine.disease
Coronary Vessels
Immunohistochemistry
Receptor
Endothelin B
Endothelin 1
Endocrinology
cardiovascular system
Female
Autopsy
medicine.symptom
Tunica Intima
Cardiology and Cardiovascular Medicine
Endothelin receptor
business
Vasoconstriction
circulatory and respiratory physiology
Zdroj: Journal of Hypertension. 24:711-721
ISSN: 0263-6352
DOI: 10.1097/01.hjh.0000217854.97369.8c
Popis: The repair process at the site of injury after percutaneous coronary intervention (PCI) is dominated by neointimal formation composed mainly of smooth muscle cells (SMC). Endothelin-1 (ET-1) is a powerful vasoconstrictor and SMC mitogen. Endothelin-converting enzyme (ECE) is the final key enzyme of endothelin processing. The effects of ET-1 are mediated by binding to endothelin type A (ETA) and endothelin type B (ETB) receptors. The ligand/receptor/ligand-producing system (ET system) could be involved in the pathogenesis of neointimal formation in humans.Fifteen post-PCI sites obtained at autopsy and eight atherectomy specimens obtained from restenotic sites were investigated using immunohistochemical single and double staining techniques. Frozen sections were stained with antibodies against ECE, ET-1, ETA and ETB receptors, SMC, macrophages and endothelial cells.At the early stage, less than 3 months after PCI, neointimal SMC were positive for ECE, ET-1, ETA and ETB receptors. The expression of ECE, ET-1, ETA and ETB receptors in these neointimal SMC decreased markedly from 6 months onwards. The ECE, ET-1, ETA and ETB receptor-positive cell areas were significantly (P0.005) greater in the first 3 months after PCI compared with 6 months or more after PCI. Atherectomy specimens also showed similar positivity.These observations strongly suggest that the expression of ECE, ET-1, ETA and ETB receptors is enhanced in neointimal SMC at early stages after PCI injury in human coronary arteries. The increased expression of the ET system may contribute to SMC proliferation/migration and vasoconstriction in human post-PCI coronary lesions.
Databáze: OpenAIRE
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