The exogenous application of AtPGLR, an endo-polygalacturonase, triggers pollen tube burst and repair

Autor: Aline Voxeur, Jean-Claude Mollet, Valérie Lefebvre, Corinne Pau-Roblot, Arnaud Lehner, Ludivine Hocq, Jean-Marc Domon, Sophie Guinand, Wafae Tabi, Josip Safran, Jérôme Pelloux, Françoise Fournet, Serge Pilard, Olivier Habrylo
Přispěvatelé: Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Arabidopsis thaliana
Amaryllis belladonna
Arabidopsis
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Plant Science
Pollen Tube
medicine.disease_cause
01 natural sciences
Plant Roots
Cell wall
03 medical and health sciences
chemistry.chemical_compound
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Pollen
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Genetics
medicine
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Pollen tube tip
Elongation
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Pectinase
[SDV.BDD]Life Sciences [q-bio]/Development Biology
ComputingMilieux_MISCELLANEOUS
biology
Arabidopsis Proteins
Lateral root
Callose
food and beverages
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Cell Biology
Oligogalacturonides
biology.organism_classification
Plants
Genetically Modified
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Cell biology
[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
030104 developmental biology
Polygalacturonase
chemistry
Saccharomycetales
Pectins
Pollen tube
010606 plant biology & botany
Zdroj: Plant Journal
Plant Journal, Wiley, 2020, 103 (2), pp.617-633. ⟨10.1111/tpj.14753⟩
Plant Journal, 2020, 103 (2), pp.617-633. ⟨10.1111/tpj.14753⟩
ISSN: 1365-313X
0960-7412
Popis: International audience; Plant cell wall remodeling plays a key role in the control of cell elongation and differentiation. In particular, fine-tuning of the degree of methylesterification of pectins was previously reported to control developmental processes as diverse as pollen germination, pollen tube elongation, emergence of primordia or elongation of dark-grown hypocotyls. However, how pectin degradation can modulate plant development has remained elusive. Here we report the characterization of a polygalacturonase (PG), AtPGLR, the gene for which is highly expressed at the onset of lateral root emergence in Arabidopsis. Due to gene compensation mechanisms, mutant approaches failed to determine the involvement of AtPGLR in plant growth. To overcome this issue, AtPGLR has been expressed heterologously in the yeast Pichia pastoris and biochemically characterized. We showed that AtPGLR is an endo-PG that preferentially releases non-methylesterified oligogalacturonides with a short degree of polymerization (< 8) at acidic pH. The application of the purified recombinant protein on Amaryllis pollen tubes, an excellent model for studying cell wall remodeling at acidic pH, induced abnormal pollen tubes or cytoplasmic leakage in the subapical dome of the pollen tube tip, where non-methylesterified pectin epitopes are detected. Those leaks could either be repaired by new β-glucan deposits (mostly callose) in the cell wall or promoted dramatic burst of the pollen tube. Our work presents the full biochemical characterization of an Arabidopsis PG and highlights the importance of pectin integrity in pollen tube elongation.
Databáze: OpenAIRE
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