Development and Validation of a New High-Performance Liquid Chromatography Method for the Simultaneous Quantification of Coenzyme Q10, Phosphatidylserine, and Vitamin C from a Cutting-Edge Liposomal Vehiculization
| Autor: | Anna Nardi-Ricart, Marta Ruiz-Garcia, Daniel Maspoch, Sonia García-Jimeno, Marc Suñé-Pou, Mary Cano-Sarabia, Josep M. Suñé-Negre, Débora Mercadé-Frutós, Encarna García-Montoya, Pilar Pérez-Lozano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Vitamin
Coenzyme Q10 Liposome Chromatography Vitamin C General Chemical Engineering Coenzims Liquid chromatography Coenzymes General Chemistry Phosphatidylserine High-performance liquid chromatography Cromatografia de líquids Article chemistry.chemical_compound Chemistry chemistry Organic compounds Solvents QD1-999 Nutrition |
| Zdroj: | ACS Omega, Vol 4, Iss 22, Pp 19710-19715 (2019) Dipòsit Digital de la UB Universidad de Barcelona Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Recercat. Dipósit de la Recerca de Catalunya instname Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) ACS Omega |
| ISSN: | 2470-1343 |
| Popis: | This is an open access article published under an ACS AuthorChoice License. See Standard ACS AuthorChoice/Editors' Choice Usage Agreement - https://pubs.acs.org/page/policy/authorchoice_termsofuse.html A high-performance liquid chromatography (HPLC) method was developed to simultaneously quantify three widely used active substances such as coenzyme Q10, phosphatidylserine, and vitamin C. This new method optimizes current timing and costs in the analyses of these three active substances. Additionally, since the analyzed compounds were encapsulated on a cutting-edge liposomal formulation, further processing was necessary to be developed prior to HPLC analyses. The technique was studied and adequately validated in accordance with the guidelines of the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) regarding selectivity, linearity, accuracy, precision, and robustness. After data treatment of results, linear regressions for all active substances showed an optimal linearity with a correlation coefficient of >0.999 in the concentration range between 70 to 130% of the liposomal formulation and less than a 3% relative standard deviation (RSD) in accuracy and precision. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|