The role of the acquisition methods in the analysis of the non-steroidal anti-inflammatory drugs in Danube River by gas chromatography - mass spectrometry
| Autor: | Ibolya Molnár-Perl, A. Helenkár, A. Sebok, Gy. Záray, A. Vasanits-Zsigrai |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Detection limit
Ketoprofen Naproxen Diclofenac Chromatography Calibration curve Anti-Inflammatory Agents Non-Steroidal Analytical chemistry Ibuprofen Gas Chromatography-Mass Spectrometry Analytical Chemistry Standard curve chemistry.chemical_compound Rivers chemistry Limit of Detection medicine Gas chromatography Gas chromatography–mass spectrometry Derivatization Water Pollutants Chemical medicine.drug |
| Zdroj: | Talanta. 82:600-607 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2010.05.014 |
| Popis: | In this paper authors describe a GC-MS acquisition study, relating to the most common, non-steroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen, naproxen, ketoprofen and diclofenac. As novelties to the field, for the trimethylsilyl (TMS) oxime ester derivatives of NSAIDs, at first, a tandem mass spectrometric (MS/MS) acquisition method has been developed, and, also for the first time, the three acquisition techniques, the full scan (FS), the selective ion monitoring (SIM) and the currently optimized MS/MS ones, have been compared: all three in parallel, under strictly the same derivatization/instrumental conditions, both from model solutions and from the Danube River samples. Critical evaluation of the three acquisition protocols was collated on their analytical performances and validated with the same characteristics like the six point calibration curve, the relative standard deviation percentages (RSD%) of parallel tests, the limit of quantitation (LOQ) and the instrumental limit of quantitation (ILQ) values. Data of six point calibration (r(2) >= 0.997) and RSD% (average: 5.8 RSD%) values proved to be independent on the acquisition methods, while, LOQ and ILQ values furnished considerable differences. Decreasing LOQ data, (expressed in ng/L concentrations) were obtained in the FS, SIM, MS/MS line for ibuprofen (1.0, 0.43, 0.41), naproxen (1.1, 1.0, 0.42), ketoprofen (2.6, 1.0, 0.49) and diclofenac (1.4. 0.41, 0.21), respectively. The same trend was determined in terms of the ILQ values. The practical utility of the optimized MS/MS technique was confirmed by the quantitation of the NSAID contents of the Danube River samples, determined by all three acquisition techniques. Results obtained confirmed the primary importance of the MS/MS acquisition method, even in comparison to the SIM one: avoiding the extreme overestimation of the ibuprofen (approximate to 100%) and ketoprofen (approximate to 400%) concentrations in the Danube River samples. (C) 2010 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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