Osteogenic properties of human myogenic progenitor cells
| Autor: | Shirabe Shimizu, Rieko Umeda, Ikuya Nonaka, Tohru Kiyono, Shigeru Yasumoto, Naohiro Hashimoto, Masayo Inagawa-Ogashiwa, Yu-ichi Goto, Michiko R. Wada |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
rho GTP-Binding Proteins
Embryology Satellite Cells Skeletal Muscle Cellular differentiation Bone Morphogenetic Protein 2 Down-Regulation Core Binding Factor Alpha 1 Subunit Biology Muscle Development Bone morphogenetic protein MyoD Bone morphogenetic protein 2 Osteogenesis Transforming Growth Factor beta Humans Progenitor cell MyoD Protein Osteoblasts Myogenesis Stem Cells PAX7 Transcription Factor Cell Differentiation Molecular biology Cell biology RUNX2 Bone Morphogenetic Proteins Stem cell Signal Transduction Developmental Biology |
| Zdroj: | Mechanisms of Development. 125:257-269 |
| ISSN: | 0925-4773 |
| DOI: | 10.1016/j.mod.2007.11.004 |
| Popis: | Here, we identified human myogenic progenitor cells coexpressing Pax7, a marker of muscle satellite cells and bone-specific alkaline phosphatase, a marker of osteoblasts, in regenerating muscle. To determine whether human myogenic progenitor cells are able to act as osteoprogenitor cells, we cultured both primary and immortalized progenitor cells derived from the healthy muscle of a nondystrophic woman. The undifferentiated myogenic progenitors spontaneously expressed two osteoblast-specific proteins, bone-specific alkaline phosphatase and Runx2, and were able to undergo terminal osteogenic differentiation without exposure to an exogenous inductive agent such as bone morphogenetic proteins. They also expressed the muscle lineage-specific proteins Pax7 and MyoD, and lost their osteogenic characteristics in association with terminal muscle differentiation. Both myoblastic and osteoblastic properties are thus simultaneously expressed in the human myogenic cell lineage prior to commitment to muscle differentiation. In addition, C3 transferase, a specific inhibitor of Rho GTPase, blocked myogenic but not osteogenic differentiation of human myogenic progenitor cells. These data suggest that human myogenic progenitor cells retain the capacity to act as osteoprogenitor cells that form ectopic bone spontaneously, and that Rho signaling is involved in a critical switch between myogenesis and osteogenesis in the human myogenic cell lineage. |
| Databáze: | OpenAIRE |
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