Embryo-scale epithelial buckling forms a propagating furrow that initiates gastrulation

Autor: Fierling, Julien, John, Alphy, Delorme, Barthélémy, Torzynski, Alexandre, Blanchard, Guy, Lye, Claire M, Popkova, Anna, Malandain, Grégoire, Sanson, Benedicte, Étienne, Jocelyn, Marmottant, Philippe, Quilliet, Catherine, Rauzi, Matteo
Přispěvatelé: Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), University of Cambridge [UK] (CAM), Morphologie et Images (MORPHEME), Inria Sophia Antipolis - Méditerranée (CRISAM), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut de Biologie Valrose (IBV), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Signal, Images et Systèmes (Laboratoire I3S - SIS), Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), John, Alphy [0000-0003-1319-3799], Blanchard, Guy [0000-0002-3689-0522], Malandain, Grégoire [0000-0002-4559-1575], Sanson, Benedicte [0000-0002-2782-4195], Étienne, Jocelyn [0000-0002-1866-5604], Marmottant, Philippe [0000-0001-6828-6656], Rauzi, Matteo [0000-0002-6313-0668], Apollo - University of Cambridge Repository, Etienne, Jocelyn, COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Blanchard, Guy B [0000-0002-3689-0522], Sanson, Bénédicte [0000-0002-2782-4195]
Rok vydání: 2022
Předmět:
animal structures
Embryo
Nonmammalian
[SDV]Life Sciences [q-bio]
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
631/1647/328/2237
General Physics and Astronomy
Embryonic Development
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
General Biochemistry
Genetics and Molecular Biology
[PHYS.MECA.MEMA]Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph]
[PHYS.MECA.MEMA] Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph]
[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Morphogenesis
Animals
[PHYS.MECA.MEFL] Physics [physics]/Mechanics [physics]/Fluid mechanics [physics.class-ph]
[PHYS.MECA.MEFL]Physics [physics]/Mechanics [physics]/Fluid mechanics [physics.class-ph]
Cell Shape
Multidisciplinary
[PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Gastrulation
639/766/747
article
631/136/1660/2127
[SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis
General Chemistry
Actomyosin
[SDV.BDD.MOR] Life Sciences [q-bio]/Development Biology/Morphogenesis
631/1647/2204
Drosophila melanogaster
embryonic structures
Drosophila
Zdroj: Nature Communications
Nature Communications, 2022, 13 (1), pp.3348. ⟨10.1038/s41467-022-30493-3⟩
Nature Communications, Nature Publishing Group, 2022, 13 (1), pp.3348. ⟨10.1038/s41467-022-30493-3⟩
ISSN: 2041-1723
DOI: 10.17863/cam.86428
Popis: Cell apical constriction driven by actomyosin contraction forces is a conserved mechanism during tissue folding in embryo development. While much is now understood of the molecular mechanism responsible for apical constriction and of the tissue-scale integration of the ensuing in-plane deformations, it is still not clear if apical actomyosin contraction forces are necessary or sufficient per se to drive tissue folding. To tackle this question, we use the Drosophila embryo model system that forms a furrow on the ventral side, initiating mesoderm internalization. Past computational models support the idea that cell apical contraction forces may not be sufficient and that active or passive cell apico-basal forces may be necessary to drive cell wedging leading to tissue furrowing. By using 3D computational modelling and in toto embryo image analysis and manipulation, we now challenge this idea and show that embryo-scale force balance at the tissue surface, rather than cell-autonomous shape changes, is necessary and sufficient to drive a buckling of the epithelial surface forming a furrow which propagates and initiates embryo gastrulation.
Databáze: OpenAIRE
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