A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity
| Autor: | Ricardo Mir, Henk-jan Schoonbeek, José León, Matteo Binda, Floriane L’Haridon, Francine Balet, Jean-Pierre Métraux, Mario Serrano, Eliane Abou-Mansour, Angélique Besson-Bard, Olivier Lamotte, Stephane Hess |
|---|---|
| Přispěvatelé: | Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Universite de Fribourg, Biotechnology and Biological Sciences Research Council, Universitat Politècnica de València (UPV), Department of Biology, Northern Arizona University [Flagstaff] |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Cutinase Arabidopsis thaliana Long chain fatty acid coenzyme A ligase Carboxy-Lyases Arabidopsis Plant Science Dry deposition 01 natural sciences Oxalic acid Hormone synthesis Superoxides Atrboh D Atrboh F lcsh:QH301-705.5 Abscisic acid Plant disease chemistry.chemical_classification Fungal protein Immunoassay Innate immunity 0303 health sciences Plant leaf food and beverages cutinase gene 3. Good health Protein secretion Botrytis Transgenic plant Cuticle Lacs2.3 protein Immunology BDG protein Arabidopsis Biosynthesis Microbiology Botryotinia fuckeliana Article 03 medical and health sciences Membrane Lipids Cutine Genetics Animal experiment Biology Molecular Biology Plant Diseases Arabidopsis protein hydrogen-peroxide Arabidopsis Proteins Fluorescent dye Oxalate decarboxylase Wound fungi Plant Pathology Hydrogen peroxide Sclerotinia-sclerotiorum Oxygen chemistry Enzyme Mutation Parasitology Gene expression lcsh:RC581-607 Protein synthesis Vegetable protein appressorium formation Unclassified drug Physiology [SDV]Life Sciences [q-bio] Diphenyliodonium salt Protein function chemistry.chemical_compound Botrytis cinerea oxidative stress F-SP-PISI botrytis-cinerea abscisic-acid sclerotinia-sclerotiorum arabidopsis-thaliana magnaporthe-grisea Carboxylyase Bdg protein Trametes biology Superoxide Abscisic-acid Membrane lipid Catalase Plants Genetically Modified Abscisic acid 3 Abscisic acid 2 Reporter gene Luminol Research Article lcsh:Immunologic diseases. Allergy Trametes versicolor Fungal Proteins Plant immunity Phytohormone Virology Coenzyme A Ligases 030304 developmental biology Reactive oxygen species Mutant biology.organism_classification Nonhuman Plant Leaves Metabolism lcsh:Biology (General) Cell permeabilization biology.protein LACS2 protein Arabidopsis Reactive oxygen metabolite Controlled study 010606 plant biology & botany |
| Zdroj: | Plos Pathogens 7 (7), e1002148 ; 1-17. (2011) RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Digital.CSIC. Repositorio Institucional del CSIC PLoS Pathogens PLoS Pathogens, Public Library of Science, 2011, 7 (7), pp.e1002148 ; 1-17. ⟨10.1371/journal.ppat.1002148⟩ PLoS Pathogens, Vol 7, Iss 7, p e1002148 (2011) |
| ISSN: | 1553-7366 1553-7374 |
| DOI: | 10.1371/journal.ppat.1002148⟩ |
| Popis: | Wounded leaves of Arabidopsis thaliana show transient immunity to Botrytis cinerea, the causal agent of grey mould. Using a fluorescent probe, histological staining and a luminol assay, we now show that reactive oxygen species (ROS), including H2O2 and O2 2, are produced within minutes after wounding. ROS are formed in the absence of the enzymes Atrboh D and F and can be prevented by diphenylene iodonium (DPI) or catalase. H2O2 was shown to protect plants upon exogenous application. ROS accumulation and resistance to B. cinerea were abolished when wounded leaves were incubated under dry conditions, an effect that was found to depend on abscisic acid (ABA). Accordingly, ABA biosynthesis mutants (aba2 and aba3) were still fully resistant under dry conditions even without wounding. Under dry conditions, wounded plants contained higher ABA levels and displayed enhanced expression of ABA-dependent and ABA-reporter genes. Mutants impaired in cutin synthesis such as bdg and lacs2.3 are already known to display a high level of resistance to B. cinerea and were found to produce ROS even when leaves were not wounded. An increased permeability of the cuticle and enhanced ROS production were detected in aba2 and aba3 mutants as described for bdg and lacs2.3. Moreover, leaf surfaces treated with cutinase produced ROS and became more protected to B. cinerea. Thus, increased permeability of the cuticle is strongly linked with ROS formation and resistance to B. cinerea. The amount of oxalic acid, an inhibitor of ROS secreted by B. cinerea could be reduced using plants over expressing a fungal oxalate decarboxylase of Trametes versicolor. Infection of such plants resulted in a faster ROS accumulation and resistance to B. cinerea than that observed in untransformed controls, demonstrating the importance of fungal suppression of ROS formation by oxalic acid. Thus, changes in the diffusive properties of the cuticle are linked with the induction ROS and attending innate defenses. This work was made possible by funds to JPM from the Swiss National Science Foundation (grant 125370). Jose´ Le´on was supported by Ministerio de Ciencia e Innovacion of Spain (BIO2008-00839) and Ricardo Mir by a FPU Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript |
| Databáze: | OpenAIRE |
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