Evaluating the effectiveness of gel formulation of irradiated seed lectin Cratylia mollis during bone repair in rats
| Autor: | Mauricélia Firmino de Santana, Maria Tereza dos Santos Correia, Ralph Santos-Oliveira, Luana Cassandra Breitenbach Barroso Coelho, Nicodemos Teles de Pontes Filho, Maria Helena Madruga Lima-Ribeiro, Carmelita de Lima Bezerra Cavalcanti, Ana Maria dos Anjos Carneiro-Leão, Adriana Ferreira Cruz |
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| Rok vydání: | 2013 |
| Předmět: |
Loose connective tissue
medicine.medical_specialty Chemistry natural products Regeneration (biology) cramoll-1 4 Stimulation Context (language use) Bone healing Bone tissue Surgery irradiated gel medicine.anatomical_structure In vivo Bone repair medicine Original Article Implant Biomedical engineering |
| Zdroj: | International Journal of Applied and Basic Medical Research |
| ISSN: | 2229-516X |
| Popis: | Context: Regeneration corresponds to the replacement of damaged cells with ones that have the same morphology and function. For experimental evaluation of materials that may favor the process of bone healing, defects are created with dimensions that prevent spontaneous regeneration. For the development and use of new drugs, it is necessary to study its effects in vitro, which depends on the formulation, concentration, and rate of irradiation in vivo and the route and frequency of administration; thus, it is possible to characterize the physiological and molecular mechanisms involved in the response and cellular effects. Objective: The objective of this study was to assess the effect of Cramoll-1,4 on the process of bone repair. Materials and Methods: A formulation of biopharmaceutical lectin Cramoll-1,4 at a concentration of 300 mg/100 mL was applied in a single application via gamma radiation and its effect on the process of bone repair in rats was assessed. Results: Histologically, it was observed that the bone defect is coated by loose connective tissue rich in fibroblasts, providing a range similar to the thick bone original and competing with site of new bone formation. This prevented direct contact between the formulation and experimental bone tissue, as, despite its proven effectiveness in experiments on the repair of skin lesions, the formulation used did not promote bone stimulation that would have promoted the tissue repair process. Conclusion: Because of the direct interference of loose tissue repair that prevented direct contact of the implant with the bone interface, the formulation did not promote bone stimulation. |
| Databáze: | OpenAIRE |
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