[Investigation of Pneumocystis jirovecii colonization in patients with pulmonary diseases]
| Autor: | Çiler Akisü, Songül Bayram Delibaş, Ahmet Emin Erbaycu, Soykan Özkoç, Ceren Erguden |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male medicine.medical_specialty Grocott's methenamine silver stain Pneumocystis carinii Gastroenterology Azure Stains Polymerase Chain Reaction Sensitivity and Specificity Giemsa stain Internal medicine parasitic diseases Bronchoscopy medicine Prevalence Pneumocystis jirovecii Humans Colonization Fluorescent Antibody Technique Indirect Lung medicine.diagnostic_test biology business.industry Pneumonia Pneumocystis General Medicine Middle Aged biology.organism_classification medicine.disease Staining Pneumonia Bronchoalveolar lavage medicine.anatomical_structure Female business Bronchoalveolar Lavage Fluid |
| Zdroj: | Turkiye parazitolojii dergisi. 38(4) |
| ISSN: | 2146-3077 |
| Popis: | OBJECTIVE The detection of Pneumocystis jirovecii or its DNA in respiratory samples from individuals who do not have signs or symptoms of pneumonia has been defined as colonization. In this study, we aimed to investigate the prevalence of P. jirovecii colonization in patients with various lung diseases. METHODS Thirty patients who were followed-up and who had undergone bronchoscopy for diagnosis of different underlying diseases or pulmonary signs were included in the study. Bronchoalveolar lavage (BAL) fluids of these patients were analyzed with nPCR amplification of the mt-LSUrRNA gene of P. jirovecii. In addition to nPCR, giemsa, Gomori's methenamine silver (GMS), and indirect fluorescence antibody (IFA) staining assays were applied to all samples. RESULTS P. jirovecii DNA was detected in 21 of 30 (70%) BAL samples by nPCR. However, P. jirovecii cysts were found in 1 of 21 nPCR-positive samples by giemsa and GMS. IFA assay showed six samples to be positive, but only four of them were found to be positive by nPCR. CONCLUSION Result of our study showed that prevalence of P. jirovecii colonization is particularly high in patients with chronic pulmonary diseases, and nPCR was a good assay for evaluation of the colonization of P. jirovecii. |
| Databáze: | OpenAIRE |
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