Role of genetic variants of Vitamin D receptor, Toll-like receptor 2 and Toll-like receptor 4 in extrapulmonary tuberculosis
| Autor: | Bilal Ahmad Wani, Rajni Singh, Dil Afroze, Sonaullah Shah, Mohmad Iqbal Mir, Ajaz Nabi Koul, Faheem Shehjar, Muzamil Farooq, Adfar Yusuf |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Tuberculosis Genotype 030106 microbiology Population Single-nucleotide polymorphism Polymorphism Single Nucleotide Microbiology Calcitriol receptor vitamin D deficiency Mycobacterium tuberculosis 03 medical and health sciences Gene Frequency medicine Humans Genetic Predisposition to Disease Risk factor education education.field_of_study biology business.industry medicine.disease biology.organism_classification Toll-Like Receptor 2 Toll-Like Receptor 4 030104 developmental biology Infectious Diseases Case-Control Studies Immunology Receptors Calcitriol business |
| Zdroj: | Microbial Pathogenesis. 156:104911 |
| ISSN: | 0882-4010 |
| Popis: | Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis (MTB). Vitamin D deficiency and vitamin D receptor (VDR) gene abnormalities confer susceptibility to tuberculosis. Toll-like receptors (TLRs), such as TLR-2, are also important mediators of inflammatory response against Mycobacterium tuberculosis. We evaluated VDR, TLR-2 and TLR-4 gene polymorphisms in patients with extrapulmonary tuberculosis (EPTB).To find out a possible association of Vitamin D receptor (VDR) (rs731236), TLR-2 (196-174 Ins Del) and TLR-4 (Thr399Ile) gene polymorphisms with extrapulmonary tuberculosis in ethnic Kashmiri population.A total of 100 extrapulmunary tuberculosis cases and 102 healthy controls were analyzed for Vitamin D receptor (VDR) (rs731236), TLR-2 (196-174 ins del) and TLR-4 (Thr399Ile) gene polymorphisms using PCR-RFLP and Allele-Specific PCR methods.We found increased frequency of TLR-4 Thr/Ile heterozygous genotype in cases as compared with healthy controls (22% vs 5.8%). Thus acting as a risk factor for extrapulmonary tuberculosis, as was elucidated from statistical analysis [OR, 4.5; 95% CI (1.74-11.68); P 0.001]. In case of TLR-2 (196-174 ins del) we observed significant differences in the homozygous variant (Del/Del) genotype of cases and controls (28% in cases2.94% in controls). Thus, TLR-2 (Del/Del) genotype acts as a strong risk factor for extrapulmonary tuberculosis predisposition [OR, 12.2; 95% CI (3.5-42.69); P 0.001]. We did not find any significant differences in the genotypic distribution of (VDR) (rs731236) T C SNP between cases and controls (P 0.05).TLR-4 (Thr/Ile) and TLR-2 (Del/Del) act as significant risk factors for extrapulmonary tuberculosis predisposition in ethnic Kashmiri population. |
| Databáze: | OpenAIRE |
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