RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis
| Autor: | Eduardo G. Gusmao, Henrike Johanna Gothe, Christian Becker, Argyris Papantonis, Nadine Übelmesser, Natasa Josipovic, Shu Zhang, Vassilis Roukos, Michael F. Chiang, Davide Marenduzzo, Janine Altmüller, Kerstin S. Wendt, Giada Forte, Adriaan B. Houtsmuller, Johan A. Slotman |
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| Přispěvatelé: | Erasmus MC other, Pathology, Cell biology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
RNA polymerase II
Chromosome conformation capture 03 medical and health sciences 0302 clinical medicine Gene expression Genetics Mitosis 030304 developmental biology Cohesin loading 0303 health sciences Multidisciplinary biology Chemistry Chromatin binding SciAdv r-articles Cell Biology Cell cycle Chromatin Cell biology Premature chromosome condensation biology.protein Interphase Biomedicine and Life Sciences Technology Platforms G1 phase 030217 neurology & neurosurgery Research Article |
| Zdroj: | Science Advances Science advances, 7(43):eabg8205. American Association for the Advancement of Science |
| ISSN: | 2375-2548 |
| Popis: | Description Following exit from mitosis, RNA Pol II is required for reestablishing 3D genome folding by loading cohesin complexes onto DNA. Mammalian chromosomes are three-dimensional entities shaped by converging and opposing forces. Mitotic cell division induces marked chromosome condensation, but following reentry into the G1 phase of the cell cycle, chromosomes reestablish their interphase organization. Here, we tested the role of RNA polymerase II (RNAPII) in this transition using a cell line that allows its auxin-mediated degradation. In situ Hi-C showed that RNAPII is required for both compartment and loop establishment following mitosis. RNAPs often counteract loop extrusion, and in their absence, longer and more prominent loops arose. Evidence from chromatin binding, super-resolution imaging, and in silico modeling allude to these effects being a result of RNAPII-mediated cohesin loading upon G1 reentry. Our findings reconcile the role of RNAPII in gene expression with that in chromatin architecture. |
| Databáze: | OpenAIRE |
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