Multitargeting activity of miR-24 inhibits long-term melatonin anticancer effects
| Autor: | Federica Mori, Matteo Pallocca, Frauke Goeman, Maria Ferraiuolo, Claudio Pulito, Etleva Korita, Andrea Sacconi, Giovanni Blandino, Paola Muti, Sabrina Strano, Maurizio Fanciulli, Raffaela Santoro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
p53 DNA repair DNA damage p38 mitogen-activated protein kinases Heterogeneous Nuclear Ribonucleoprotein A1 miR-24 melatonin Antineoplastic Agents Apoptosis Breast Neoplasms Biology Bioinformatics law.invention Melatonin MiR-24 P53 PML RNA-Seq Oncology 03 medical and health sciences Downregulation and upregulation law medicine Biomarkers Tumor Tumor Cells Cultured Humans Gene Cell Proliferation Cell growth Prognosis Survival Rate MicroRNAs 030104 developmental biology Case-Control Studies Colonic Neoplasms Cancer research Suppressor Female medicine.drug Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | We have previously shown that melatonin exerts tumor suppressor activities by inducing the p38-p53 axis. This occurred within a few hours while no data are available on how melatonin pathway can be sustained on the long term. Here we show that miR-24, which has been demonstrated to target genes involved in the DNA repair process, targets p38, p53, PML and H2AX simultaneously. We show that long-term treatment with melatonin can decrease miR-24 levels post-transcriptionally, which pairs with a long-wave regulation of genes involved in cell proliferation, DNA damage, RNA metabolism and cell shape and transformation. Moreover, we show that melatonin can inhibit cell proliferation and migration, at least in part, by downregulating miR-24. Furthermore, we propose the involvement of hnRNP A1, which is downregulated by melatonin and involved in miRNA processing, in the regulation of miR-24 levels by melatonin. We conclude showing that miR-24 is upregulated in colon, breast and head and neck datasets and its levels negatively correlate with overall survival. |
| Databáze: | OpenAIRE |
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