A Polymerase Chain Reaction-based Assay for Diagnosing Varicella-zoster Virus Retinitis in Patients With Acquired Immunodeficiency Syndrome
| Autor: | Baruch D. Kuppermann, Graham Short, Daniel F. Martin, Todd P. Margolis, Alexander R. Irvine, Diane Chandler |
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| Rok vydání: | 1997 |
| Předmět: |
Herpesvirus 3
Human Pathology medicine.medical_specialty Time Factors Opportunistic infection viruses Retinitis Progressive outer retinal necrosis Biology medicine.disease_cause Herpes Zoster Polymerase Chain Reaction Sensitivity and Specificity Virus Herpesviridae law.invention Chickenpox law Alphaherpesvirinae medicine Humans Polymerase chain reaction Acquired Immunodeficiency Syndrome integumentary system Varicella zoster virus virus diseases Retinal Necrosis Syndrome Acute medicine.disease biology.organism_classification Virology medicine.infectious_disease Ophthalmology DNA Viral |
| Zdroj: | American Journal of Ophthalmology. 123:157-164 |
| ISSN: | 0002-9394 |
| DOI: | 10.1016/s0002-9394(14)71031-1 |
| Popis: | Purpose To develop a rapid, sensitive, and specific laboratory assay based on the polymerase chain reaction for the diagnosis of varicella-zoster virus retinitis in patients with acquired immunodeficiency syndrome (AIDS). Methods We developed and tested a polymerase chain reaction-based assay for the detection of varicella-zoster virus DNA in vitreous samples. We attempted to detect varicella-zoster virus DNA in 14 vitreous samples from patients with AIDS and a clinical diagnosis of progressive outer retinal necrosis syndrome. For controls, we also attempted to detect varicella-zoster virus DNA in vitreous samples from 75 immunocompetent patients with vitreoretinal disease and 88 patients with AIDS and vitreoretinal inflammatory disease not related to progressive outer retinal necrosis syndrome. Results Varicella-zoster virus DNA was detected in 11 of 14 vitreous samples from AIDS patients with progressive outer retinal necrosis syndrome. All three samples that scored negative for varicella-zoster virus DNA came from eyes that had been treated aggressively with antiviral drugs and had clinically inactive disease at the time of vitreous biopsy. Varicella-zoster virus DNA was detected in only two of 75 control vitreous samples from immunocompetent patients with vitreoretinal disease and two of 88 control vitreous samples from patients with AIDS and vitreoretinal inflammatory disease not related to progressive outer retinal necrosis syndrome. Conclusion We have developed a rapid, sensitive, and specific polymerase chain reaction-based diagnostic assay for varicella-zoster virus DNA that will assist in the diagnosis of varicella-zoster virus retinitis in patients with AIDS. |
| Databáze: | OpenAIRE |
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