Multiciliated cell basal bodies align in stereotypical patterns coordinated by the apical cytoskeleton
| Autor: | Hatsuho Kanoh, Kazuhiro Tateishi, Daisuke Taniguchi, Sachiko Tsukita, Elisa Herawati, Shuji Ishihara |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
congenital hereditary and neonatal diseases and abnormalities Time Factors Cell Survival Cell Green Fluorescent Proteins Biology Models Biological Article Polymerization 03 medical and health sciences chemistry.chemical_compound Basal (phylogenetics) 0302 clinical medicine Imaging Three-Dimensional Microtubule medicine Basal body Animals Computer Simulation Cilia Cytoskeleton Process (anatomy) Tomography Research Articles Nocodazole Calcium-Binding Proteins Viscoelastic fluid Cell Polarity Cell Differentiation Epithelial Cells Cell Biology Anatomy Actins Basal Bodies Cell biology Mice Inbred C57BL Trachea 030104 developmental biology medicine.anatomical_structure chemistry Cell Tracking 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Herawati et al. developed a long-term and high-resolution live imaging system for cultured mouse tracheal multiciliated cells. Using both experimental and theoretical studies, they reveal the developmental principle of ciliary basal body alignment directed by apical cytoskeletons. Multiciliated cells (MCCs) promote fluid flow through coordinated ciliary beating, which requires properly organized basal bodies (BBs). Airway MCCs have large numbers of BBs, which are uniformly oriented and, as we show here, align linearly. The mechanism for BB alignment is unexplored. To study this mechanism, we developed a long-term and high-resolution live-imaging system and used it to observe green fluorescent protein–centrin2–labeled BBs in cultured mouse tracheal MCCs. During MCC differentiation, the BB array adopted four stereotypical patterns, from a clustering “floret” pattern to the linear “alignment.” This alignment process was correlated with BB orientations, revealed by double immunostaining for BBs and their asymmetrically associated basal feet (BF). The BB alignment was disrupted by disturbing apical microtubules with nocodazole and by a BF-depleting Odf2 mutation. We constructed a theoretical model, which indicated that the apical cytoskeleton, acting like a viscoelastic fluid, provides a self-organizing mechanism in tracheal MCCs to align BBs linearly for mucociliary transport. |
| Databáze: | OpenAIRE |
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