Unveiling the biodiversity of lipid species in Corynebacteria- characterization of the uncommon lipid families in C. glutamicum and pathogen C. striatum by mass spectrometry
| Autor: | Nicholas K. Goldner, Raju V. V. Tatituri, Hay Yan J. Wang, Gautam Dantas, Fong-Fu Hsu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization Corynebacterium Biochemistry Corynebacterium glutamicum Mycolic acid Diglycerides 03 medical and health sciences chemistry.chemical_compound Lipidomics Cardiolipin Humans Cytidine diphosphate Diacylglycerol kinase chemistry.chemical_classification 030102 biochemistry & molecular biology biology Chemistry Phosphatidylglycerols General Medicine biology.organism_classification Lipid Metabolism Lipids Trehalose dimycolate 030104 developmental biology Cord Factors lipids (amino acids peptides and proteins) |
| Zdroj: | Biochimie. 178 |
| ISSN: | 1638-6183 |
| Popis: | Uncommon lipids in biotechnologically important Corynebacterium glutamicum and pathogen Corynebacterium striatum in genus Corynebacterium are isolated and identified by linear ion-trap multiple stage mass spectrometry (LIT MSn) with high resolution mass measurement. We redefined several lipid structures that were previously mis-assigned or not defined, including cytidine diphosphate diacylglycerol (CDP-DAG), glucuronosyl diacylglycerol (GlcA-DAG), (α- d -mannopyranosyl)-(1 → 4)-(α-D-glucuronyl diacyglycerol (Man-GlcA-DAG), 1-mycolyl-2-acyl-phosphatidylglycerol (MA-PG), acyl trehalose monomycolate (acyl-TMM). We also report the structures of mycolic acid, phosphatidylglycerol, phosphatidylinositol, cardiolipin, trehalose dimycolate lipids in which many isomeric structures are present. The LIT MSn approaches afford identification of the functional group, the fatty acid substituents and their regiospecificity in the molecules, revealing the biodiversities of the lipid species in two Corynebacterium strains that have played very different and important roles in human nutrition and health. |
| Databáze: | OpenAIRE |
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