A novel structure seen when foot-and-mouth disease virus-induced poly (U) polymerase acts in a cell-free system
| Autor: | J. Polatnick, S.H. Wool |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Poly U
Deoxyribonucleases biology Cell-Free System RNase P Poly U polymerase RNA Templates Genetic biology.organism_classification Molecular biology Nucleotidyltransferases Cell-free system Microscopy Electron Aphthovirus Ribonucleases biology.protein Poly A-U Anatomy Foot-and-mouth disease virus Poly A Molecular Biology Polymerase RNA Double-Stranded |
| Zdroj: | Journal of ultrastructure research. 80(3) |
| ISSN: | 0022-5320 |
| Popis: | A novel structure was seen during the cell-free synthesis of poly (U) by foot-and-mouth disease virus-induced poly (U) polymerase acting on a poly (A) template. An electron-microscopy spreading procedure using benzyldimethylalkylammonium chloride visualized a double-stranded RNA product containing sets of open regions where single-stranded poly (U) may be made. A doublestranded poly (A)·poly (U) structure resistant to RNase A is assumed to form during the initial 15 min of poly (U) polymerase action. Apparently partial unwinding of the double-stranded structure then occurs followed by synthesis of single-stranded poly (U) in the series of newly opened regions. |
| Databáze: | OpenAIRE |
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