Evaluation of sample handling in relation to levels of tissue inhibitor of metalloproteinases-1 measured in blood by immunoassay
| Autor: | M.N. Holten-Andersen, A.-S. Schrohl, N. Brünner, H.J. Nielsen, C.K. Høgdall, E.V.S. Høgdall |
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| Rok vydání: | 2003 |
| Předmět: |
0301 basic medicine
Immunoassay Cancer Research Time Factors Tissue Inhibitor of Metalloproteinase-1 Clinical Biochemistry Temperature Enzyme-Linked Immunosorbent Assay Pathology and Forensic Medicine Specimen Handling 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology 030220 oncology & carcinogenesis Neoplasms Freezing Biomarkers Tumor Humans Female Edetic Acid Chelating Agents |
| Zdroj: | The International journal of biological markers. 18(3) |
| ISSN: | 0393-6155 |
| Popis: | Background The possible effect of preanalytical conditions such as blood sample preparation and handling on TIMP-1 levels in blood needs thorough investigation. Materials and Methods Blood was collected in dry tubes and tubes containing EDTA and kept at 4°C or 20°C for 1, 3, 8, 24 or 72 hours before processing into serum or EDTA plasma. In addition, serum and EDTA plasma samples were frozen and thawed 1–8 times. TIMP-1 was measured by ELISA. Results Time to processing for up to 72 hours did not significantly affect TIMP-1 levels in serum. In EDTA plasma, TIMP-1 levels were stable for up to eight hours; however, if samples were kept for 24 hours or longer the TIMP-1 levels increased (pConclusions Handling and processing of blood samples is crucial for TIMP-1 measurement by immunoassay. In serum, TIMP-1 levels are unaffected by time to processing. Plasma samples should be processed within eight hours to avoid a TIMP-1 increase. For the measurement of TIMP-1 in archival material, serum should not be used because TIMP-1 levels are significantly affected by repeated freezing and thawing; archival plasma can readily be used provided that samples have not been frozen and thawed more than six times. |
| Databáze: | OpenAIRE |
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