Bright far-red fluorescent protein for whole-body imaging
| Autor: | Ekaterina A. Bogdanova, Konstantin A. Lukyanov, Elena A. Solovieva, Sergey Lukyanov, Arkady F. Fradkov, Dmitriy M. Chudakov, Galina V. Ermakova, Dmitry Shcherbo, Andrey G. Zaraisky, Ekaterina M. Merzlyak, Tatiana V. Chepurnykh |
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| Rok vydání: | 2007 |
| Předmět: |
Brightness
Embryo Nonmammalian genetic structures Light penetration Whole body imaging Biology Biochemistry Xenopus laevis Microscopy Fluorescence Resonance Energy Transfer Fluorescent protein Animals Humans Emission spectrum Transgenes Molecular Biology Fluorescent Dyes Microscopy Confocal Far-red Cell Biology Fluorescence Luminescent Proteins Microscopy Fluorescence Biophysics Biotechnology HeLa Cells |
| Zdroj: | Nature methods. 4(9) |
| ISSN: | 1548-7091 |
| Popis: | For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum. |
| Databáze: | OpenAIRE |
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