Differential behaviour of normal, transformed and Fanconi's anemia lymphoblastoid cells to modeled microgravity
| Autor: | Monica Sancandi, Paolo Degan, Francesca Barbieri, Silvia Viaggi, Paola Cuccarolo |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
DNA damage
Endocrinology Diabetes and Metabolism Clinical Biochemistry lcsh:Medicine Apoptosis Biology medicine.disease_cause Jurkat cells Thiobarbituric Acid Reactive Substances Jurkat Cells Adenosine Triphosphate Fanconi anemia medicine Humans Pharmacology (medical) Lymphocytes Molecular Biology Cell Line Transformed Cell Proliferation Biochemistry medical Membrane Potential Mitochondrial Analysis of Variance Cell growth Weightlessness Lymphoblast Research lcsh:R Biochemistry (medical) Deoxyguanosine General Medicine Cell Biology medicine.disease Cell biology Fanconi Anemia Glucose Cell culture 8-Hydroxy-2'-Deoxyguanosine Poly(ADP-ribose) Polymerases Carcinogenesis Energy Metabolism |
| Zdroj: | Journal of Biomedical Science Journal of Biomedical Science, Vol 17, Iss 1, p 63 (2010) |
| ISSN: | 1423-0127 |
| Popis: | Background Whether microgravity might influence tumour growth and carcinogenesis is still an open issue. It is not clear also if and how normal and transformed cells are differently solicited by microgravity. The present study was designed to verify this issue. Methods Two normal, LB and HSC93, and two transformed, Jurkat and 1310, lymphoblast cell lines were used as representative for the two conditions. Two lymphoblast lines from Fanconi's anemia patients group A and C (FA-A and FA-C, respectively), along with their isogenic corrected counterparts (FA-A-cor and FA-C-cor) were also used. Cell lines were evaluated for their proliferative ability, vitality and apoptotic susceptibility upon microgravity exposure in comparison with unexposed cells. Different parameters correlated to energy metabolism, glucose consumption, mitochondrial membrane potential (MMP), intracellular ATP content, red-ox balance and ability of the cells to repair the DNA damage product 8-OHdG induced by the treatment of the cells with 20 mM KBrO3 were also evaluated. Results Transformed Jurkat and 1310 cells appear resistant to the microgravitational challenge. On the contrary normal LB and HSC93 cells display increased apoptotic susceptibility, shortage of energy storages and reduced ability to cope with oxidative stress. FA-A and FA-C cells appear resistant to microgravity exposure, analogously to transformed cells. FA corrected cells did shown intermediate sensitivity to microgravity exposure suggesting that genetic correction does not completely reverts cellular phenotype. Conclusions In the light of the reported results microgravity should be regarded as an harmful condition either when considering normal as well as transformed cells. Modeled microgravity and space-based technology are interesting tools in the biomedicine laboratory and offer an original, useful and unique approach in the study of cellular biochemistry and in the regulation of metabolic pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|