Virulence gene transcription, phylogroups, and antibiotic resistance of cervico-vaginal pathogenic E. coli in Mexico

Autor: Ernesto Arturo Rojas Jiménez, Jaime Bustos-Martínez, Felipe Vaca-Paniagua, Sergio Vaca, Luis Rey García Cortés, Tania Hernández-Jaimes, Eric Monroy-Pérez, Gloria Luz Paniagua-Contreras, Nancy Nolasco Alonso, Pablo Domínguez-Trejo, Aida Hamdan-Partida, Areli Bautista Cerón
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Bacterial Diseases
0301 basic medicine
Transcription
Genetic
Artificial Gene Amplification and Extension
Cervix Uteri
Drug resistance
Pathology and Laboratory Medicine
medicine.disease_cause
Polymerase Chain Reaction
law.invention
Antibiotics
law
Genotype
Medicine and Health Sciences
Phylogeny
Polymerase chain reaction
Multidisciplinary
Virulence
Antimicrobials
Drugs
Drug Resistance
Microbial
Bacterial Pathogens
Infectious Diseases
Experimental Organism Systems
Medical Microbiology
Urinary Tract Infections
Vagina
Prokaryotic Models
Medicine
Female
Pathogens
Research Article
Escherichia
Urology
Science
DNA transcription
030106 microbiology
Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Model Organisms
Antibiotic resistance
Enterobacteriaceae
Microbial Control
Multiplex polymerase chain reaction
Genetics
Escherichia coli
medicine
Humans
Molecular Biology Techniques
Molecular Biology
Microbial Pathogens
Mexico
Gene
Pharmacology
Bacteria
Gut Bacteria
Organisms
Biology and Life Sciences
Escherichia Coli Infections
030104 developmental biology
Antibiotic Resistance
Animal Studies
Antimicrobial Resistance
Gene expression
Zdroj: PLoS ONE, Vol 15, Iss 6, p e0234730 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: The pathogenicity of Escherichia coli strains that cause cervico-vaginal infections (CVI) is due to the presence of several virulence genes. The objective of this study was to define the variability regarding the genotype of antibiotic resistance, the transcription profiles of virulence genes after in vitro infection of the vaginal cell line A431 and the phylogroup composition of a group of cervico-vaginal E. coli strains (CVEC). A total of 200 E. coli strains isolated from Mexican women with CVI from two medical units of the Mexican Institute of Social Security were analysed. E. coli strains and antibiotic resistance genes were identified using conventional polymerase chain reaction (PCR), and phylogroups were identified using multiplex PCR. Virulence gene transcription was measured through reverse-transcriptase real-time PCR after infection of the vaginal cell line A431. The most common antibiotic resistance genes among the CVEC strains were aac(3)II, TEM, dfrA1, sul1, and qnrA. The predominant phylogroup was B2. The genes most frequently transcribed in these strains were fimH, papC, irp2, iroN, kpsMTII, cnf1, and ompT, mainly in CVEC strains isolated from chronic and occasional vaginal infections. The strains showed a large diversity of transcription of the virulence genes phenotype and antibiotic resistance genotype, especially in the strains of phylogroups, B2, A, and D. The strains formed 2 large clusters, which contained several subclusters. The genetic diversity of CVEC strains was high. These strains have a large number of transcription patterns of virulence genes, and one-third of them carry three to seven antibiotic resistance genes.
Databáze: OpenAIRE
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