A new regulatory element mediates ethanol repression of KlADH3, a Kluyveromyces lactis gene coding for a mitochondrial alcohol dehydrogenase
| Autor: | Ivana Fantozzi, Cristina Mazzoni, Claudia Getuli, Claudio Falcone, Michele Saliola |
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| Rok vydání: | 2007 |
| Předmět: |
Molecular Sequence Data
Mutagenesis (molecular biology technique) Applied Microbiology and Biotechnology Microbiology Fungal Proteins Mitochondrial Proteins chemistry.chemical_compound Kluyveromyces In vivo Gene Expression Regulation Fungal Promoter Regions Genetic Psychological repression Gene Alcohol dehydrogenase Kluyveromyces lactis Ethanol biology Base Sequence Alcohol Dehydrogenase General Medicine biology.organism_classification Culture Media Mitochondria Enhancer Elements Genetic Biochemistry chemistry Transcriptional repression biology.protein Mutagenesis Site-Directed Gene Deletion |
| Zdroj: | FEMS yeast research. 7(5) |
| ISSN: | 1567-1356 |
| Popis: | KlADH3 is a Kluyveromyces lactis alcohol dehydrogenase gene induced in the presence of all respiratory carbon sources except ethanol, which specifically represses this gene. Deletion analysis of the KlADH3 promoter revealed the presence of both positive and negative elements. However, by site-directed mutagenesis and gel retardation experiments, we identified a 15-bp element responsible for the transcriptional repression of this gene by ethanol. In particular, this element showed putative sites required for the sequential binding of ethanol-induced factors responsible for the repressed conditions, and the binding of additional factors relieved repression. In addition, we showed that the ethanol element was required for in vivo repression of KlAdh3 activity. |
| Databáze: | OpenAIRE |
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