Effects of exogenous recombinant human granulocyte colony-stimulating factor (filgrastim, rhG-CSF) on neutrophils of critically ill patients with systemic inflammatory response syndrome depend on endogenous G-CSF plasma concentrations on admission
| Autor: | Britta Lasch, Britt Harms-Schirra, Sami Voglic, Kristoffel R. Dumon, Ingrid Lorenz, W. Gross-Weege, Manfred Weiss, Elisabeth Marion Schneider |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Filgrastim Neutrophils medicine.drug_class Granulocyte Critical Care and Intensive Care Medicine Immunostimulant Sepsis Leukocyte Count Phagocytosis Risk Factors Intensive care Granulocyte Colony-Stimulating Factor Humans Medicine Prospective Studies Infusions Intravenous Aged Respiratory Burst Multiple Trauma business.industry Receptors IgG Length of Stay Middle Aged Flow Cytometry medicine.disease Recombinant Proteins Systemic Inflammatory Response Syndrome Respiratory burst Granulocyte colony-stimulating factor Systemic inflammatory response syndrome Treatment Outcome medicine.anatomical_structure Surgical Procedures Operative Immunology Female business medicine.drug |
| Zdroj: | Intensive Care Medicine. 29:904-914 |
| ISSN: | 1432-1238 0342-4642 |
| DOI: | 10.1007/s00134-003-1734-y |
| Popis: | To investigate the effects of exogenous recombinant human granulocyte colony-stimulating factor (rhG-CSF; filgrastim) application on the neutrophils of patients at risk of sepsis following major trauma or operation.Randomized controlled trial.Surgical intensive care unit and research laboratory of a university hospital.Twenty-seven patients with systemic inflammatory response syndrome (SIRS).Thirteen patients were treated with filgrastim (1 micro g.kg.24 h) for 10 days as a continuous infusion. Fourteen patients served as controls.Surface expression of FcgammaR type I (CD64), phagocytosis of E. coli, and the E. coli-induced oxidative burst of neutrophils were tested by flow cytometry. On the first postoperative/posttraumatic day, endogenous G-CSF plasma concentrations were300 pg/ml in seven controls (subgroup 1) and nine filgrastim patients (subgroup 3), and were already elevated with500 pg/ml in seven controls (subgroup 2) and four filgrastim patients (subgroup 4). G-CSF values ( P=0.0026, subgroup 1/3; P=0.0167, 2/4), neutrophil counts ( P=0.0026, 1/3; P=0.0167, 2/4), and CD64 expression ( P=0.0013, 1/3) were higher in filgrastim-treated than non-treated subgroups, but not phagocytic and burst activities. From day zero to day 1, phagocytosis decreased in subgroups 1 (5/7 patients) and 3 (5/9), but increased in subgroups 2 (5/7) and 4 (3/4), and respiratory burst activity decreased in subgroup 3 (8/9).Besides activation of neutrophil maturation, low-dose rhG-CSF application in postoperative patients with SIRS has different effects on neutrophil functions, in part depending on already endogenously produced G-CSF. |
| Databáze: | OpenAIRE |
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