Fast, Efficient, and Targeted Liposome Delivery Mediated by DNA Hybridization
| Autor: | Mark Loznik, Qing Liu, Jan Willem de Vries, Xintong Yang, Robert Göstl, Andreas Herrmann, Bart J Crielaard, Hongyan Li, Zhuojun Meng |
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| Rok vydání: | 2019 |
| Předmět: |
Cell
Biomedical Engineering Pharmaceutical Science 02 engineering and technology 010402 general chemistry Endocytosis 01 natural sciences Biomaterials chemistry.chemical_compound Drug Delivery Systems Complementary DNA medicine Humans ddc:610 Liposome Chemistry DNA–DNA hybridization Cell Membrane Nucleic Acid Hybridization DNA 021001 nanoscience & nanotechnology Lipids 0104 chemical sciences Membrane medicine.anatomical_structure Liposomes Drug delivery Biophysics 0210 nano-technology HeLa Cells |
| Zdroj: | Advanced Healthcare Materials Advanced healthcare materials 8(14), 1900389 (2019). doi:10.1002/adhm.201900389 |
| ISSN: | 2192-2640 |
| DOI: | 10.1002/adhm.201900389 |
| Popis: | Safety and efficacy, two significant parameters in drug administration, can be improved by site-specific delivery approaches. Here a fast, efficient, and targeted liposome delivery system steered by a DNA hybridization recognition mechanism is presented. For this purpose, lipid-terminated DNA is inserted in both liposome and cell membranes by simple mixing of the components. Cellular accumulation of cargo encapsulated in the liposomal core is substantially enhanced when the DNA sequence on the cell is complementary to that on the liposome. Additionally, in mixed cell populations, liposomes discriminate targets by their complementary DNA sequences. Exposure of cells to low temperature and endocytosis inhibitors suggests a caveolae-dependent endocytosis uptake pathway. Mechanistically, hybridization between DNA strands spatially traps liposomes and cell membranes in close proximity, consequently increases the local liposome concentration, and thereby enhances cellular uptake of liposomes and their payload. This programmable delivery system might contribute to new applications in molecular biology and drug delivery. |
| Databáze: | OpenAIRE |
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