Expression of a prostate-associated protein, human glandular kallikrein (hK2), in breast tumours and in normal breast secretions
| Autor: | Angeliki Magklara, Christina V. Obiezu, Margot H. Black, Donald J.A. Sutherland, D J Tindall, Michael A. Levesque, C Y F Young, Eleftherios P. Diamandis, Edward R. Sauter |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
medicine.medical_specialty medicine.medical_treatment Mammary gland Breast Neoplasms Receptors Cell Surface Biology kallikrein gene family Breast cancer breast cancer Prostate Internal medicine Progesterone receptor medicine Tumor Cells Cultured Humans Breast Human Glandular Kallikrein Regular Article Prostate-Specific Antigen medicine.disease prostate cancer human glandular kallikrein Steroid hormone Prostate-specific antigen medicine.anatomical_structure Endocrinology Oncology Inhalation Nipples Female Kallikreins Breast carcinoma |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | The recent demonstration of human glandular kallikrein (hK2) expression in a breast carcinoma cell line has suggested that this putatively prostate-restricted, steroid hormone-regulated protease may also be expressed in breast epithelium in vivo and secreted into the mammary duct system. Given that the only substrate yet identified for hK2 activity is the precursor of prostate-specific antigen (PSA), the expression of which in breast carcinomas may be associated with favourable prognosis, our purpose was to examine the expression pattern of both hK2 and PSA in breast tumour tissues. Cytosolic extracts of 336 primary breast carcinomas prepared for routine oestrogen receptor (ER) and progesterone receptor (PR) analysis, as well as 31 nipple aspirates from six women with non-diseased mammary glands, were assayed for hK2 and PSA using immunofluorometric assays developed by the authors. In the tumour extracts, measurable hK2 and PSA concentrations were detected in 53% and 73% of cases respectively, and were positively correlated to each other (r = 0.59, P = 0.0001). Higher concentrations of PSA and hK2 were found in tumours expressing steroid hormone receptors (P = 0.0001 for PSA and P = 0.0001 for hK2, by Wilcoxon tests for both ER and PR), and both PSA (r = 0.25, P = 0.0001) and hK2 (r = 0.22, P = 0.0001) correlated directly with PR levels. A negative correlation between patient age and PSA (r = –0.12, P = 0.03) was also found. Both proteins were present in nipple aspirate fluid at relatively high concentrations which were positively correlated (r = 0.53, P = 0.002). The molecular weights of the immunoreactive species quantified by the hK2 and PSA assays were established by high-performance liquid chromatography (HPLC) and were consistent with the known molecular weights of hK2 and PSA. Together these data provide the first evidence, to our knowledge, that both malignant breast tissue and normal breast secretion contain measurable quantities of hK2, and that the degree of hK2 expression or secretion is directly proportional to the expression of PSA and steroid hormone receptors. hK2 expression may therefore be a marker of steroid hormone action in breast tissue. © 2000 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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