Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)
| Autor: | Natalia Olivares, Patricio Hinrichsen, Carlos Aguirre, Evelyn Sánchez |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Naupactus cervinus Citrus Naupactus Biology Real-Time Polymerase Chain Reaction 01 natural sciences Sensitivity and Specificity law.invention 03 medical and health sciences law TaqMan Animals Multiplex Chile Polymerase chain reaction 030304 developmental biology 0303 health sciences Ecology Weevil General Medicine biology.organism_classification Coleoptera 010602 entomology Horticulture Real-time polymerase chain reaction Insect Science Curculionidae Weevils Multiplex Polymerase Chain Reaction |
| Zdroj: | Journal of economic entomology. 114(1) |
| ISSN: | 1938-291X |
| Popis: | Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus. This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/μl to 1 × 10-6 pg/μl, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification. |
| Databáze: | OpenAIRE |
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