Angiogenic response to passive movement and active exercise in individuals with peripheral arterial disease
| Autor: | Ylva Hellsten, Birgitte Hoier, Philip J. Walker, Madla A. Passos, Anita Green, Christopher D. Askew, Jens Bangsbo, Meegan Walker |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Microdialysis Physiology Arterial disease Neovascularization Physiologic Active exercise Angiopoietin-2 Thrombospondin 1 Peripheral Arterial Disease chemistry.chemical_compound Physiology (medical) Internal medicine medicine Humans RNA Messenger Muscle Skeletal Exercise Aged Vascular Endothelial Growth Factor Receptor-1 business.industry Skeletal muscle Articles Middle Aged Intermittent claudication Surgery Peripheral Vascular endothelial growth factor medicine.anatomical_structure chemistry Case-Control Studies Cardiology Female medicine.symptom business |
| Zdroj: | Journal of Applied Physiology. 115:1777-1787 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/japplphysiol.00979.2013 |
| Popis: | Peripheral arterial disease (PAD) is caused by atherosclerosis and is associated with microcirculatory impairments in skeletal muscle. The present study evaluated the angiogenic response to exercise and passive movement in skeletal muscle of PAD patients compared with healthy control subjects. Twenty-one PAD patients and 17 aged control subjects were randomly assigned to either a passive movement or an active exercise study. Interstitial fluid microdialysate and tissue samples were obtained from the thigh skeletal muscle. Muscle dialysate vascular endothelial growth factor (VEGF) levels were modestly increased in response to either passive movement or active exercise in both subject groups. The basal muscle dialysate level of the angiostatic factor thrombospondin-1 protein was markedly higher ( P < 0.05) in PAD patients compared with the control subjects, whereas soluble VEGF receptor-1 dialysate levels were similar in the two groups. The basal VEGF protein content in the muscle tissue samples was ∼27% lower ( P < 0.05) in the PAD patients compared with the control subjects. Analysis of mRNA expression for a range of angiogenic and angiostatic factors revealed a modest change with active exercise and passive movement in both groups, except for an increase ( P < 0.05) in the ratio of angiopoietin-2 to angiopoietin-1 mRNA in the PAD group with both interventions. PAD patients and aged individuals showed a similar limited angiogenic response to active exercise and passive movement. The limited increase in muscle extracellular VEGF combined with an elevated basal level of thrombospondin-1 in muscle extracellular fluid of PAD patients may restrict capillary growth in these patients. |
| Databáze: | OpenAIRE |
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