Heterogeneity of VP4 neutralization epitopes among serotype P1A human rotavirus strains
| Autor: | R S Tamez, Luis Padilla-Noriega, Susana López, Harry B. Greenberg, Griselda Menchaca, Juan F. Contreras, Carlos F. Arias |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Microbiology (medical)
Serotype Diarrhea Rotavirus viruses Clinical Biochemistry Immunology medicine.disease_cause Antibodies Viral Epitope Neutralization Microbiology Epitopes fluids and secretions Capsid Antigen Neutralization Tests medicine Immunology and Allergy Humans Typing Serotyping Neutralizing antibody biology virus diseases Infant Virology Gastroenteritis Child Preschool biology.protein Capsid Proteins medicine.symptom Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | We have used serotype-specific VP4 and VP7 neutralizing monoclonal antibodies (Nt-MAbs), as well as subgroup (SG)-specific MAbs, to characterize by enzyme immunoassay rotavirus strains isolated from diarrheic infants in the city of Monterrey, Mexico, from July 1993 to March 1994. Of a total of 465 children studied, 140 were rotavirus positive, including 3 patients infected with non-group A rotaviruses. The SG and VP7 (G) serotype specificities could be determined for 118 (84%) of the 140 rotavirus-positive stool specimens; 4 rotavirus strains were serotype G1 and SGII; 1 strain was serotype G2 and SGI+II; 112 strains were serotype G3 and SGII; 1 strain was serotype G3 and SGI; and none of the strains was serotype G4. Fifty-eight specimens, representing the 13 different group A rotavirus electropherotypes detected, were chosen for VP4 (P) serotyping. Of these, 48 (83%) strains reacted with the P1A serotype-specific Nt-MAb 1A10. None of the strains reacted with the serotype P2-specific Nt-MAbs tested. Not all viruses that reacted with Nt-MAb 1A10 were recognized by Nt-MAbs 2A3 and 2G1, which also recognize P1A strains, indicating heterogeneity of neutralization epitopes among serotype P1A human rotaviruses. This heterogeneity could be relevant for the specificity of the VP4-mediated neutralizing antibody immune response and indicates the need for antigenic characterization, in addition to genomic typing, of the VP4 proteins of circulating human rotavirus field strains. |
| Databáze: | OpenAIRE |
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