Analysis of close proximity quenching of phosphorescent metalloporphyrin labels in oligonucleotide structures
| Autor: | Paul J. O'Sullivan, Dmitri B. Papkovsky, Martina Burke, Dmitri V. Yashunsky, Gelii V. Ponomarev |
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| Rok vydání: | 2007 |
| Předmět: |
Porphyrins
Metalloporphyrins Molecular Sequence Data Oligonucleotides chemistry.chemical_element Photochemistry Biochemistry Chemistry Techniques Analytical Analytical Chemistry chemistry.chemical_compound Environmental Chemistry Spectroscopy Platinum Quenching Luminescent Agents Base Sequence Oligonucleotide Nucleic Acid Hybridization DNA Resonance (chemistry) Porphyrin chemistry Luminescent Measurements Oligonucleotide Probes Phosphorescence Palladium |
| Zdroj: | Analytica Chimica Acta. 585:139-146 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2006.12.025 |
| Popis: | Quenching of phosphorescent platinum(II) and palladium(II) coproporphyrin (MeCP) labelled oligonucleotides was investigated. Strong hybridization-specific quenching was observed in duplex DNA structures with a variety of quenchers and with two identical porphyrin labels when in close proximity. Classical resonance energy transfer mechanism was ruled out, since quenching did not correlate with spectral overlaps and lifetime changes were insignificant. Quenching of MeCP by the free quenchers in solution revealed that porphyrin-porphyrin quenching is predominantly static while other dyes quench dynamically. The results suggest that the quenching in DNA duplex proceeds via direct contact. |
| Databáze: | OpenAIRE |
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