Intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model
Autor: | Paweł Buszman, Piotr P. Buszman, Andrzej Bochenek, Joanna Fryc-Stanek, Jack L. Martin, Krzysztof Milewski, Dariusz Szurlej, Jacek Pająk, Wojciech Wojakowski, Michał Jelonek, Szymon Wiernek, Michael E Spurlock, Wanda Jackiewicz, Marcin Dębiński |
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Rok vydání: | 2010 |
Předmět: |
Male
medicine.medical_specialty Pathology Time Factors Sus scrofa Myocardial Infarction Infarction Myocardial Reperfusion Myocardial Reperfusion Injury chemistry.chemical_compound Internal medicine Genetics Medicine Animals Myocardial infarction Evans Blue Ejection fraction Adiponectin biology business.industry General Medicine medicine.disease Troponin medicine.anatomical_structure chemistry biology.protein Cardiology Female business Reperfusion injury Artery |
Zdroj: | International journal of molecular medicine. 27(6) |
ISSN: | 1791-244X |
Popis: | Reperfusion injury (RI) remains an important limitation of myocardial revascularization. The aim of the present study was to evaluate the influence of the intracoronary injection of adiponectin on RI and cardiomyocyte death in a porcine myocardial infarction model. Acute infarction in 14 Polish domestic pigs was induced by inflation of an over the wire balloon (OTW) catheter in the medial left anterior descending artery for 60 min. The study group consisted of 7 pigs in which intracoronary adiponectin (50 µg) was infused through the OTW catheter immediately before reperfusion. The control group (n=7) was administered placebo. Animals were sacrificed after two days of follow-up. The infarct area (IA) was stained with tetrazoline and the area at risk (AAR) with intracoronary administration of Evans Blue dye before euthanasia. Hearts in each group had similar AARs (46.2±9.9% vs. 48.4±6.2% of the whole myocardium, p=ns). The IA/AAR% and IA were smaller in the study group when compared to the control (24.7±4.0% vs. 45.3±22.5%, p=0.005; and 11.7±4.9% vs. 20.5±5.6%, p=0.01, respectively). These outcomes corresponded well with the peak troponin levels after 12 h (109.9±60.9 ng/ml vs. 185.5±39.4 ng/ml, p=0.017). After two days there was a significantly higher LVEF in the study group (51.4±8.5% vs. 33.9±8.6%, p=0.002). There was also a trend toward lower apoptosis enhancement in the viable myocardium in the study group (3.11±2.3 vs. 8.92±6.3; p=0.07). The administration of adiponectin into the infarct- related artery is safe and feasible. The treatment significantly reduced the infarct size. |
Databáze: | OpenAIRE |
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