Thermogemmata fonticola gen. nov., sp. nov., the first thermophilic planctomycete of the order Gemmatales from a Kamchatka hot spring
| Autor: | Stepan V. Toshchakov, Olga L. Kovaleva, Elizaveta A. Bonch-Osmolovskaya, Olga A. Podosokorskaya, Alexander G. Elcheninov, Andrei A. Novikov, Ilya V. Kublanov |
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| Rok vydání: | 2020 |
| Předmět: |
DNA
Bacterial Applied Microbiology and Biotechnology Microbiology Hot Springs Russia 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Botany Primary nutritional groups Yeast extract Ecology Evolution Behavior and Systematics Phylogeny 030304 developmental biology 0303 health sciences Base Composition biology Strain (chemistry) Bacteria 030306 microbiology Thermophile Fatty Acids Planctomycetes Vitamin K 2 Sequence Analysis DNA Galactan biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques chemistry |
| Zdroj: | Systematic and applied microbiology. 44(1) |
| ISSN: | 1618-0984 |
| Popis: | A novel aerobic moderately thermophilic bacterium, designated strain 2918T, was isolated from a terrestrial hot spring of Kamchatka, Russian Federation. Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by budding. The strain grew at 25-60°C and within a pH range of 5.0-8.0 with an optimum at 54-60°C and pH 7.5. Strain 2918T did not require sodium chloride or yeast extract for growth. It was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (starch, lichenan, galactan, arabinan, xanthan gum, beta-glucan). No growth was observed under anaerobic conditions neither in the presence of sulfur, nitrate, or thiosulfate nor without adding any electron acceptor. Major cellular fatty acids were C18:0 and C20:0. The respiratory quinone was MK-6. The size of the genome of strain 2918T was 4.81 Mb. Genomic DNA G+C content was 60.4mol%. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 2918T represented a distinct lineage of the order Gemmatales within Planctomycetes. Based on phylogenetic analysis and phenotypic features, the novel isolate was assigned to a novel genus in the Gemmatales for which the name Thermogemmata gen. nov. is proposed. Strain 2918T (=KCTC 72012T =VKM B-3161T) represents its first species Thermogemmata fonticola sp. nov. |
| Databáze: | OpenAIRE |
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