DKK4 enhances resistance to chemotherapeutics 5-Fu and YN968D1 in colorectal cancer cells
| Autor: | Xuan-Fu Xu, Jie Shen, Nanhua Hu, Sheng-li He, Jin Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology chemotherapy resistance Cancer Research medicine.medical_specialty Colorectal cancer Cell colorectal cancer Biology 03 medical and health sciences 0302 clinical medicine dickkopf-related protein 4 5-fluorouracil Internal medicine medicine Viability assay Oncogene Cancer Articles Cell cycle medicine.disease 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction Cell culture 030220 oncology & carcinogenesis Cancer research YN968D1 |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2016.5461 |
| Popis: | Dickkopf-related protein 4 (DKK4) is a target of the β-catenin/transcription factor 4 complex in colorectal cancer. Previous research has demonstrated that its expression level may vary and has indicated that it may have a role in the development of resistance to chemotherapy in colorectal cancer cells. In the present study, DKK4 was over expressed in several colorectal cancer cell lines. The DKK4 over-expressing cell lines were screened using reverse transcription quantitative polymerase chain reaction analysis and western blotting. Analysis of cell viability in the control and DKK4 over-expressing cell lines, following treatment with 5-fluorouracil (5-Fu), YN968D1 or both, indicated that DKK4 over-expressing cells exhibit increased drug resistance. The results of Transwell chamber assays suggested that DKK4 had an effect on cell migration. Furthermore, the results from flow cytometric analysis showed that the percentage of apoptotic cells was reduced in the DKK4 over-expressing cell lines, following drug treatment, compared with the control. The present data suggested that DKK4 may enhance the resistance of colorectal cancer cells to 5-Fu and YN968D1 treatment, when used alone or in combination. |
| Databáze: | OpenAIRE |
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