Auxin inhibits endocytosis and promotes its own efflux from cells
| Autor: | Tomasz Paciorek, Nadia Ruthardt, York-Dieter Stierhof, Niko Geldner, Neil Emans, David A. Morris, Jiri Friml, Gerd Jürgens, Jan Petrášek, Jürgen Kleine-Vehn, Eva Zazímalová |
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| Rok vydání: | 2005 |
| Předmět: |
Auxin influx
Auxin efflux media_common.quotation_subject Gravitropism Arabidopsis Biology Endocytosis Plant Roots Auxin Tobacco heterocyclic compounds PIN proteins Internalization media_common Feedback Physiological chemistry.chemical_classification Multidisciplinary Indoleacetic Acids Arabidopsis Proteins Cell Membrane fungi food and beverages Biological Transport Cell biology chemistry Biochemistry Polar auxin transport |
| Zdroj: | Nature. 435:1251-1256 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/nature03633 |
| Popis: | One of the mechanisms by which signalling molecules regulate cellular behaviour is modulating subcellular protein translocation. This mode of regulation is often based on specialized vesicle trafficking, termed constitutive cycling, which consists of repeated internalization and recycling of proteins to and from the plasma membrane. No such mechanism of hormone action has been shown in plants although several proteins, including the PIN auxin efflux facilitators, exhibit constitutive cycling. Here we show that a major regulator of plant development, auxin, inhibits endocytosis. This effect is specific to biologically active auxins and requires activity of the Calossin-like protein BIG. By inhibiting the internalization step of PIN constitutive cycling, auxin increases levels of PINs at the plasma membrane. Concomitantly, auxin promotes its own efflux from cells by a vesicle-trafficking-dependent mechanism. Furthermore, asymmetric auxin translocation during gravitropism is correlated with decreased PIN internalization. Our data imply a previously undescribed mode of plant hormone action: by modulating PIN protein trafficking, auxin regulates PIN abundance and activity at the cell surface, providing a mechanism for the feedback regulation of auxin transport. |
| Databáze: | OpenAIRE |
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