Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
| Autor: | Niina Jääskeläinen, Tero A. H. Järvinen, Marc Cerrada-Gimenez, Giedrius Kalesnykas, Rubina Thapa, Maria Vähätupa, Hannele Uusitalo-Jarvinen |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty General Chemical Engineering 030204 cardiovascular system & hematology Retinal Neovascularization General Biochemistry Genetics and Molecular Biology Retina Neovascularization 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Retinal Diseases Ischemia Medicine Animals Hyperoxia General Immunology and Microbiology business.industry General Neuroscience Retinal Retinopathy of prematurity Diabetic retinopathy Hypoxia (medical) medicine.disease eye diseases Rats Mice Inbred C57BL Oxygen Disease Models Animal 030104 developmental biology medicine.anatomical_structure chemistry cardiovascular system sense organs medicine.symptom business Retinopathy |
| Zdroj: | Journal of visualized experiments : JoVE. (163) |
| ISSN: | 1940-087X |
| Popis: | One of the commonly used models for ischemic retinopathies is the oxygen-induced retinopathy (OIR) model. Here we describe detailed protocols for the OIR model induction and its readouts in both mice and rats. Retinal neovascularization is induced in OIR by exposing rodent pups either to hyperoxia (mice) or alternating levels of hyperoxia and hypoxia (rats). The primary readouts of these models are the size of neovascular (NV) and avascular (AVA) areas in the retina. This preclinical in vivo model can be used to evaluate the efficacy of potential anti-angiogenic drugs or to address the role of specific genes in the retinal angiogenesis by using genetically manipulated animals. The model has some strain and vendor specific variation in the OIR induction which should be taken into consideration when designing the experiments. |
| Databáze: | OpenAIRE |
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