Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes
| Autor: | Demi T. Djajadi, Anders Jensen, Anne S. Meyer, Mads Møller Jensen, Henning Jørgensen, Manuel Pinelo, Marianne Glasius, Lisbeth Garbrecht Thygesen, Marlene Oliveira |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
lcsh:Biotechnology Biomass Cellulase macromolecular substances Management Monitoring Policy and Law 01 natural sciences Applied Microbiology and Biotechnology complex mixtures Lignin lcsh:Fuel chemistry.chemical_compound Apparent surface abundance lcsh:TP315-360 010608 biotechnology Enzymatic hydrolysis lcsh:TP248.13-248.65 Organic chemistry Cellulases Biorefining Cellulose Physical barrier Inhibition biology 010405 organic chemistry Renewable Energy Sustainability and the Environment Depolymerization Research food and beverages S/G ratio β-O-4 linkage 0104 chemical sciences General Energy Corn stover chemistry biology.protein Adsorption Biotechnology |
| Zdroj: | Djajadi, D T, Jensen, M M, Oliveira, M, Jensen, A, Thygesen, L G, Pinelo, M, Glasius, M, Jørgensen, H & Meyer, A S 2018, ' Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes ', Biotechnology for Biofuels, vol. 11, no. 1, 85 . https://doi.org/10.1186/s13068-018-1085-0 Biotechnology for Biofuels Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-13 (2018) Djajadi, D T, Jensen, M M, Oliveira, M, Jensen, A, Thygesen, L G, Pinelo, M, Glasius, M, Jørgensen, H & Meyer, A S 2018, ' Lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier rather than by inducing nonproductive adsorption of enzymes ', Biotechnology for Biofuels, vol. 11, 85 . https://doi.org/10.1186/s13068-018-1085-0 |
| Popis: | Background Lignin is known to hinder efficient enzymatic conversion of lignocellulose in biorefining processes. In particular, nonproductive adsorption of cellulases onto lignin is considered a key mechanism to explain how lignin retards enzymatic cellulose conversion in extended reactions. Results Lignin-rich residues (LRRs) were prepared via extensive enzymatic cellulose degradation of corn stover (Zea mays subsp. mays L.), Miscanthus × giganteus stalks (MS) and wheat straw (Triticum aestivum L.) (WS) samples that each had been hydrothermally pretreated at three severity factors (log R0) of 3.65, 3.83 and 3.97. The LRRs had different residual carbohydrate levels—the highest in MS; the lowest in WS. The residual carbohydrate was not traceable at the surface of the LRRs particles by ATR-FTIR analysis. The chemical properties of the lignin in the LRRs varied across the three types of biomass, but monolignols composition was not affected by the severity factor. When pure cellulose was added to a mixture of LRRs and a commercial cellulolytic enzyme preparation, the rate and extent of glucose release were unaffected by the presence of LRRs regardless of biomass type and severity factor, despite adsorption of the enzymes to the LRRs. Since the surface of the LRRs particles were covered by lignin, the data suggest that the retardation of enzymatic cellulose degradation during extended reaction on lignocellulosic substrates is due to physical blockage of the access of enzymes to the cellulose caused by the gradual accumulation of lignin at the surface of the biomass particles rather than by nonproductive enzyme adsorption. Conclusions The study suggests that lignin from hydrothermally pretreated grass biomass retards enzymatic cellulose degradation by acting as a physical barrier blocking the access of enzymes to cellulose rather than by inducing retardation through nonproductive adsorption of enzymes. Electronic supplementary material The online version of this article (10.1186/s13068-018-1085-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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