Molecular rearrangement of lactose plasmid DNA associated with high-frequency transfer and cell aggregation in Lactococcus Iactis 712
| Autor: | Sumio Maeda, Simon R. Swindell, Helen M. Dodd, Michael J. Gasson |
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| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial Gene Rearrangement Base Sequence Molecular Sequence Data Lactococcus lactis Genetic transfer Biology biology.organism_classification Microbiology Lactose Factors Cell aggregation Bacterial genetics Transposition (music) F Factor chemistry.chemical_compound Plasmid chemistry Biochemistry Conjugation Genetic Lactose Molecular Biology DNA |
| Zdroj: | Molecular Microbiology. 6:3213-3223 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1992.tb01776.x |
| Popis: | High-frequency conjugation of the lactose plasmid pLP712 is associated with a constitutive cell aggregation phenotype and is facilitated by cointegration with a sex factor. Analysis of 23 independently derived enlarged lactose plasmids revealed that the sex factor DNA present in cointegrates varied in size. This suggested that more than simple cointegration with a sex factor plasmid was involved. Further analysis led to the discovery of a chromosomally located sex factor that could excise and be lost or exist as labile plasmid DNA. Cointegration with this sex factor was shown to be promoted by transposition of a copy of ISSI present on the lactose plasmid, and models are presented to account for the complex and variable structures of the resulting enlarged lactose plasmids. |
| Databáze: | OpenAIRE |
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