Maternal plasma or human serum albumin in wash buffer enhances enrichment and ex vivo expansion of human umbilical cord blood CD34+cells
Autor: | Yvonne K. Kwok, Mary Hoi Yin Tang, Yu-Lung Lau, Helen K. W. Law, Cora Suk Wai Ngai, Elizabeth T. Lau |
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Rok vydání: | 2007 |
Předmět: |
Cell Culture Techniques
Serum albumin Antigens CD34 Biology Immunophenotyping Blood cell Andrology Plasma Pregnancy Nucleated cell Blood plasma medicine Humans Progenitor cell Serum Albumin Cell Proliferation Hematopoietic Stem Cell Transplantation Hematology Fetal Blood Flow Cytometry Hematopoietic Stem Cells Human serum albumin Hematopoietic Stem Cell Mobilization Blood Cell Count Transplantation medicine.anatomical_structure Immunology biology.protein Female Fetal bovine serum medicine.drug |
Zdroj: | British Journal of Haematology. 137:468-474 |
ISSN: | 1365-2141 0007-1048 |
DOI: | 10.1111/j.1365-2141.2007.06606.x |
Popis: | Umbilical cord blood is a valuable source of haemopoietic stem/progenitor cells (HSC) for transplantation. This study explored the effect of maternal plasma/human serum albumin (HSA) in the purification and culture conditions of CD34 + cells derived from human umbilical cord blood. During CD34 + cell enrichment, including maternal plasma or HSA instead of fetal bovine serum (FBS) in the wash buffer, significantly increased the purity and the fold expansion of CD34 + cells. The increase in fold expansion of CD34 + cells was independent of CD34 + cell purity before expansion. With FBS, the mean fold expansion of CD34 + cells and total nucleated cells on day 7 was 9·7 ± 5·5 and 39·7 ± 13·7 respectively. The use of maternal plasma increased the mean fold expansion of CD34 + cells and total nucleated cells on day 7 to 28·2 ± 6·7 and 71·5 + 15·4 respectively. When HSA was added to wash buffer, the mean fold expansion of CD34 + cells and total nucleated cells were 30·4 ± 10·5 and 83·5 ± 24·8 respectively. No statistical significance was found between using HSA and maternal plasma on total cell and CD34 + cell expansion. We propose that HSA in maternal plasma was responsible for the positive effect on CD34 + cell enrichment and expansion. |
Databáze: | OpenAIRE |
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