Cell competition corrects noisy Wnt morphogen gradients to achieve robust patterning in the zebrafish embryo
| Autor: | Jumpei Nogami, Yasuyuki Ohkawa, Tohru Ishitani, Takamasa Masuda, Ryutaro Akiyoshi, Hironobu Furuie, Shizuka Ishitani, Shohei Ogamino, Yuki Akieda, Nobuyuki Shimizu |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian animal structures Science Morphogenesis General Physics and Astronomy Apoptosis SMAD urologic and male genital diseases Time-Lapse Imaging Article Morphogen signalling General Biochemistry Genetics and Molecular Biology Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Animals lcsh:Science Wnt Signaling Pathway Zebrafish beta Catenin Body Patterning Regulation of gene expression Multidisciplinary biology Cadherin Wnt signaling pathway Gene Expression Regulation Developmental General Chemistry Zebrafish Proteins biology.organism_classification Embryonic stem cell Cell biology Embryonic induction 030104 developmental biology Microscopy Fluorescence embryonic structures lcsh:Q 030217 neurology & neurosurgery Morphogen |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-17 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Morphogen signalling forms an activity gradient and instructs cell identities in a signalling strength-dependent manner to pattern developing tissues. However, developing tissues also undergo dynamic morphogenesis, which may produce cells with unfit morphogen signalling and consequent noisy morphogen gradients. Here we show that a cell competition-related system corrects such noisy morphogen gradients. Zebrafish imaging analyses of the Wnt/β-catenin signalling gradient, which acts as a morphogen to establish embryonic anterior-posterior patterning, identify that unfit cells with abnormal Wnt/β-catenin activity spontaneously appear and produce noise in the gradient. Communication between unfit and neighbouring fit cells via cadherin proteins stimulates apoptosis of the unfit cells by activating Smad signalling and reactive oxygen species production. This unfit cell elimination is required for proper Wnt/β-catenin gradient formation and consequent anterior-posterior patterning. Because this gradient controls patterning not only in the embryo but also in adult tissues, this system may support tissue robustness and disease prevention. Gradients of morphogens such as Wnt provide instructive cues for cell identities during development. Here, the authors report that in the developing zebrafish embryo, cell competition and elimination of unfit cells are required for proper Wnt gradient formation. |
| Databáze: | OpenAIRE |
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