Serum Preadipocyte Factor-1 Concentrations in Females with Obesity and Type 2 Diabetes Mellitus: The Influence of Very Low Calorie Diet, Acute Hyperinsulinemia, and Fenofibrate Treatment
| Autor: | Tomáš Roubíček, Martin Matoulek, Denisa Haluzikova, P Kaválková, P Trachta, Martin Haluzik, Milos Mraz, Daniel Novák, V. Touskova, Z Lacinová, M. Urbanova, J. Drapalova |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
endocrine system diseases Endocrinology Diabetes and Metabolism medicine.medical_treatment food.diet Clinical Biochemistry Adipose tissue Biochemistry Endocrinology food Insulin resistance Fenofibrate Hyperinsulinism Internal medicine medicine Hyperinsulinemia Humans PPAR alpha Obesity Caloric Restriction Anthropometry business.industry Insulin Leptin Body Weight Calcium-Binding Proteins Biochemistry (medical) Membrane Proteins nutritional and metabolic diseases Type 2 Diabetes Mellitus General Medicine Middle Aged medicine.disease Very low calorie diet Diabetes Mellitus Type 2 Glucose Clamp Technique Intercellular Signaling Peptides and Proteins Female business medicine.drug |
| Zdroj: | Hormone and Metabolic Research. 45:820-826 |
| ISSN: | 1439-4286 0018-5043 |
| Popis: | Appropriate differentiation capacity of adipose tissue significantly affects its ability to store lipids and to protect nonadipose tissues against lipid spillover and development of insulin resistance. Preadipocyte factor-1 (Pref-1) is an important negative regulator of preadipocyte differentiation. The aim of our study was to explore the changes in circulating Pref-1 concentrations in female subjects with obesity (OB) (n=19), females with obesity and type 2 diabetes mellitus (T2DM) (n=22), and sex- and age-matched healthy control subjects (C) (n=22), and to study its modulation by very low calorie diet (VLCD), acute hyperinsulinemia during isoglycemic-hyperinsulinemic clamp, and 3 months' treatment with PPAR-α agonist fenofibrate. At baseline, serum Pref-1 concentrations were significantly higher in patients with T2DM compared to control group, while only nonsignificant trend towards higher levels was observed in OB group. 3 weeks of VLCD decreased Pref-1 levels in both OB and T2DM group, whereas 3 months of fenofibrate treatment had no significant effect. Hyperinsulinemia during the clamp significantly suppressed Pref-1 levels in both C and T2DM subjects and this suppression was unaffected by fenofibrate treatment. In a combined population of all groups, circulating Pref-1 levels correlated positively with insulin, leptin and glucose levels and HOMA (homeostasis model assessment) index. We conclude that elevated Pref-1 concentrations in T2DM subjects may contribute to impaired adipose tissue differentiation capacity associated with insulin resistance in obese patients with T2DM. The decrease of Pref-1 levels after VLCD may be involved in the improvement of metabolic status and the amelioration of insulin resistance in T2DM patients. |
| Databáze: | OpenAIRE |
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