Microbial solvent formation revisited by comparative genome analysis
| Autor: | Klaus Winzer, Peter Dürre, Nigel P. Minton, Sharon J. Reid, Anja Poehlein, David T. Jones, Stefanie K. Flitsch, José David Montoya Solano, Preben Krabben, Edward Green, Rolf Daniel |
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| Přispěvatelé: | Department of Molecular and Cell Biology, Faculty of Science |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Clostridium acetobutylicum C. beijerinckii 030106 microbiology Management Monitoring Policy and Law Applied Microbiology and Biotechnology Genome Acetone Clostridia 03 medical and health sciences chemistry.chemical_compound Clostridium Phylogenetics Food science C. saccharobutylicum Phylogeny biology Renewable Energy Sustainability and the Environment business.industry Research Butanol biology.organism_classification C. saccharoperbutylacetonicum Biotechnology 030104 developmental biology General Energy chemistry Solvents Fermentation business Bacteria |
| Zdroj: | Biotechnology for Biofuels Biotechnol Biofuels |
| ISSN: | 1754-6834 |
| DOI: | 10.1186/s13068-017-0742-z |
| Popis: | Background Microbial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents are important feedstocks for the chemical and biofuel industry. However, biological synthesis suffers from high substrate costs and competition from chemical synthesis supported by the low price of crude oil. To render the biotechnological production economically viable again, improvements in microbial and fermentation performance are necessary. However, no comprehensive comparisons of respective species and strains used and their specific abilities exist today. Results The genomes of a total 30 saccharolytic Clostridium strains, representative of the species Clostridium acetobutylicum, C. aurantibutyricum, C. beijerinckii, C. diolis, C. felsineum, C. pasteurianum, C. puniceum, C. roseum, C. saccharobutylicum, and C. saccharoperbutylacetonicum, have been determined; 10 of them completely, and compared to 14 published genomes of other solvent-forming clostridia. Two major groups could be differentiated and several misclassified species were detected. Conclusions Our findings represent a comprehensive study of phylogeny and taxonomy of clostridial solvent producers that highlights differences in energy conservation mechanisms and substrate utilization between strains, and allow for the first time a direct comparison of sequentially selected industrial strains at the genetic level. Detailed data mining is now possible, supporting the identification of new engineering targets for improved solvent production. Electronic supplementary material The online version of this article (doi:10.1186/s13068-017-0742-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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